脂联素对流体切应力下骨髓间充质干细胞损伤的保护作用观察

Protective effect of adiponectin on bone marrow mesenchymal stem cell injury caused under fluid shear stress

目的探讨脂联素(APN)是否可减轻流体切应力(SS)对骨髓间充质干细胞(BMSCs)的损伤及其可能机制。方法采用全血贴壁法分离、培养BMSCs并进行鉴定。将BMSCs分为对照组、SS组、SS+APN组,采用平板液体流动腔模型给予BMSCs流体切应力作用24h,显微镜下观察细胞形态,TUNEL染色检测BMSCs的凋亡情况,Western blotting检测Akt、p-Akt及Bax蛋白的表达。结果与对照组比较,SS组BMSCs凋亡明显增加,Bax表达明显上调。与SS组比较,SS+APN组细胞形态明显改善,细胞增殖显著增加,Bax表达显著下调,p-Akt表达显著上调。三组Akt的表达无明显差异。结论脂联素可通过激活Akt信号通路抑制流体切应力对BMSCs的损伤。

Objective To explore whether adiponectin（APN） can attenuate the injury of bone marrow mesenchymal stem cells（BMSCs） caused under fluid shear stress（SS） and the mechanism involved. Methods BMSCs were isolated and cultured by whole blood adherent method, and then identified and divided into control group, SS（10dyne/cm2） group and SS＋APN group. BMSCs were subjected to fluid SS for 24 hours by using liquid plate flow chamber model, the cell morphology was observed by microscopy, the apoptosis of BMSCs was detected by TUNEL staining, and Western blotting was performed to detect the protein expressions of Akt, p-Akt and Bax. Results Compared with the control group, the apoptosis of BMSCs significantly increased, and the expression of Bax was up regulated in SS group. Compared with SS group, the cell morphology was significantly improved, the number of cells obviously increased, the Bax expression was down-regulated and the p-Akt expression was up-regulated markedly in SS＋APN group. There was no significant difference in Akt expression among the three groups, but the p-Akt expression significantly increased in SS＋APN pretreatment group. Conclusion Adiponectin may activate the Akt signaling pathway and inhibit the apoptosis of BMSCs under fluid SS.