主动脉弓缩窄模型小鼠肠道菌群的变化

Intestinal flora changes in a mouse model of transverse aortic constriction

目的研究主动脉弓缩窄模型(TAC)小鼠肠道菌群的变化,为预防和治疗心血管疾病寻找新靶点。方法12只C57BL/6小鼠随机分为假手术组(N组)和主动脉弓缩窄组(TN组),每组6只。TN组采用微创手术将小鼠主动脉结扎缩窄到适宜程度,N组除不结扎外其他操作与TN组相同。获取处理后22d小鼠粪便样本,通过16S r DNA测序检测其微生物组成,应用微生物生态学定量观察进行生物信息学分析。结果与N组比较,TN组小鼠粪便样本吉氏副拟杆菌属丰度增加,乳杆菌科、乳杆菌属和耳蜗形梭菌丰度降低。结论主动脉弓缩窄模型小鼠肠道菌群发生改变。肠道菌群可能是一种潜在的预防或治疗心血管疾病的靶点。

Objective To investigate intestinal microbiota changes of model mice with transverse aortic constriction（TAC） to find a new target point for preventing and treating cardiovascular disease. Methods Twelve C57BL/6 mice were randomly divided into TAC model group（group TN） and control group（group N） of 6 mice each. The group TN received minimally invasive surgery for ligating the aorta to make its constriction to the appropriate degree, while the group N received same operation but no constriction. Faecal samples were collected 22 days after the treatment. Intestinal flora were determined by 16 S r DNA pyrosequencing and bioinformatics clustering analysis were performed with software of Quantitative Insights into Microbial Ecology. Results A higher abundance of Parabacteroides and a lower abundances of Lactobacillaceae, Lactobacillus and Cocleatum were present in TAC mice compared with the controls. Conclusion Intestinal flora changes would take place in the TAC mice. Intestinal flora may be a potential target for prevention and treatment of cardiovascular diseases, but further validation should be performed to verify the relationship between those intestinal flora changes and disease progression in animal models.