摘要
目的观察骨桥蛋白(OPN)与整合素αvβ3在主动脉夹层(AD)中的表达及意义。方法雌性C57BL/6小鼠60只随机均分为两组:A组每天腹腔注射血管紧张素Ⅱ4.5mg/kg建立AD模型;B组注射生理盐水作为对照。连续注射14d后处死小鼠,观察AD形成情况,取主动脉组织行HE染色,采用免疫组织化学染色法检测OPN与整合素αvβ3的表达。结果 B组小鼠未形成AD。A组中,6只未见明显AD形成,20只小鼠形成AD,4只小鼠因AD破裂出血死亡。A组OPN和整合素αvβ3阳性表达率均高于B组(86.7%vs.6.7%和80.0%vs.13.3%)(P<0.05)。形成AD小鼠的OPN和整合素αvβ3阳性表达率均高于未形成AD小鼠(95.8%vs.13.9%和87.4%vs.19.4%)(P<0.05)。在形成AD小鼠中,OPN和整合素αvβ3表达呈正相关(rs=0.966,P<0.05)。结论形成AD的小鼠主动脉组织中,OPN与整合素αvβ3呈高表达。
Objective To study the expressions and significance of osteopontin (OPN ) and integrinαvβ3 in mice with arotic dissection (AD) .Methods Sixty C57BL/6 mice were randomly and equally divided into two groups .The mice in group A were intraperitoneally injected angiotensin Ⅱ4.5 mg/kg daily for 14 days to establish AD model .The mice in group B were injected normal saline as the controls .The mice were killed 14 days late and the aortas were taken for HE staining .The expressions of OPN and integrinαvβ3 were detected by immunohistochemistry .Results There was no AD formation in group B .Of the mice in group A ,no AD was seen in 6 mice and AD was formed in 24 mice ,of which 4 mice died of hemorrhage secondary to AD rupture .The positive expression rates of OPN and integrin αvβ3 were higher in group A than those in group B(86.7% vs .6.7% and 80.0%vs .13.3% )(P〈0 .05) ,which were higher in the mice with AD than those without AD (95.8% vs . 13.9% and 87.4% vs .19.4% ) ( P〈0 .05 ) .The positive expression rate of OPN was positively correlated with that of integrin αvβ3 in the mice with AD in group A (rs=0.966 ,P〈0 .05) .Conclusion The OPN and integrin αvβ3 in aortic tissues are highly expressed in mice with AD .
作者
李宽
陶卫东
王艳
浦小洁
贾湉
LI Kuan TAO Weidong WANG Yan et al(Department of Vascular Surgery, Kunshan Hospital of Traditional Chinese Medicine, Kunshan 215300, CHINA)
出处
《江苏医药》
CAS
2016年第20期2193-2195,F0003,共4页
Jiangsu Medical Journal