颗粒蛋白前体缺失型腹膜巨噬细胞的体外炎症应答

The in vitro inflammatory response of granular protein precursor deleted peritoneal macrophages

探讨颗粒蛋白前体缺失型巨噬细胞的体外炎症反应。选取野生型C57BL/6雄性小鼠6-8周(WT)和PGRN基因敲除雄性小鼠6-8周(KO)为实验动物。向小鼠腹腔内注射1m L6%的淀粉,脱颈法处死小鼠后提取腹膜细胞。用瑞士染色后油镜下观察细胞,用流式细胞仪进行细胞检测,显微镜下观察腹膜巨噬细胞吞噬功能,ELISA法检测腹膜巨噬细胞培养上清中TNF-a和IL-12因子。WT小鼠和PGRN基因敲除KO小鼠腹膜细胞的数目、形态和种类及巨细胞表面标志物和巨噬细胞吞噬功能均无显著性差异(p>0.05)。PGRN基因敲除KO小鼠来源腹膜巨噬细胞培养上清中前炎性因子TNF-a和IL-12的含量较WT小鼠明显增高。PGRN对腹膜细胞的数目、形态、种类和巨噬细胞表面标志物没有显著影响,但会使巨噬细胞炎症反应增强。

To investigate the in vitro inflammatory response of the granular protein precursor. Selection of wild-type 6 -8 male mice （6 -8） and PGRN （WT） C57BL/6 weeks （KO） in male mice as experimental an- imals. After intraperitoneal injection of lm16% into the mice, the mice were sacrificed and the peritoneal cells were extracted. Switzerland after staining under oil microscope to observe the cell, by flow cytometry for cell detection, under a microscope observation of peritoneal macrophage phagocytic function and ELISA method for the detection of peritoneal macrophage culture supernatant TNF-and IL-12 factor. There were no significant differences in the number, shape, type, and phagocytic function of peritoneal cells of WT mice and PGRN knockout KO miee（p 〉0. 05 ）. The contents of TNF-a and IL-12 were significantly higher in PGRN mice than in KO mice, and the contents of and WT were significantly higher in the supernatant of peritoneal macrophages. PGRN has no significant effect on the number, shape, type of peritoneal cells and the surface markers of macrophages, but it can enhance the inflammatory response of macrophages.