半枝莲对白血病K562细胞VEGF表达的影响

Effects of Scutellaria Barbata on VEGF Expression in K562 Cells

目的:探讨中药半枝莲抗K562细胞血管生成作用。方法:以半枝莲提取物(0.5、1.0、2.0和4.0 g/ml)作用于K562细胞为实验组,以生理盐水作用于K562细胞为空白对照组,在作用24、36、48 h后用MTT法检测各组对K562细胞增殖情况的影响;半枝莲作用K562细胞48 h后,用酶联免疫吸附(ELISA)法检测各实验组及空白对照组K562细胞上清液中VEGF的浓度,RT-PCR法检测K562细胞中VEGF mRNA的表达水平。结果:半枝莲提取物可以抑制K562细胞增殖,且呈一定的浓度依赖性(r=0.56);空白组K562细胞上清液中VEGF的浓度最高,在实验组VEGF浓度较在空白组中明显下降,各组间比较差异显著(P<0.05),有统计学意义;不同浓度半枝莲提取物作用于K562细胞48h后VEGF mRNA的表达均降低,目的基因与β-actin灰度比值逐渐下降,各组间差异显著(P<0.05)。结论:半枝莲提取物抑制K562细胞增殖,其作用机制可能与降低K562细胞VEGF的浓度和下调VEG F mRNA的表达有关。

Objective：To investigate the anti-angiogenesis effect of Scutellaria barbata extract（SBE） on chronic myeloid leukemia（CML） K562 cell line in vitro.Methods：The proliferating activity after treating K562 cells with 0.5,1.0,2.0 and 4.0 g/ml SB for 24,36,48 hours were assessed by MTT assay.The level of vascular endothelial growth factor（VEGF） in the culture supernatant of K562 cells was determined by ELISA;and the expression of VEGF mRNA was detected by RT-PCR.Results：MTT assay showed that SBE could inhibit the proliferation of K562 cells in a dosedependent manner（r= 0.56）;ELISA displayed that the concentration of VEGF in K562 cells in blank-control group was most high;after intervention of K562 cells by SBE（0.5,1.0,2.0 and 4.0 g/ml） for 48 h,the concentration of VEGF decreased,the comparison between different groups showed significant differences（P〈0.05）;after treatment with SBE for 48 h,the expression of VEGF mRNA in K562 cells decreased,the gray scale ratio of target gene/β-actin declined,and the difference between various groups was statistically significant（P〈0.05）.Conclution：SBE can inhibit K562 cell proliferation,its action mechanism may related with the VEGF level concentration in K562 cells and down-regulation of VEGF mRNA expression.