抗CD44单克隆抗体A3D8对HL-60细胞AP-1表达的影响

Effect of Anti-CD44 Monoclonal Antibody A3D8 on Expression of AP-1 in HL-60 Cells

目的:探讨抗CD44单克隆抗体A3D8对急性髓系白血病细胞转录因子AP-1表达的影响。方法:用A3D8干预人白血病细胞株HL-60,采用MTT法和流式细胞术检测细胞增殖、细胞周期变化;RT-PCR及Western blot检测A3D8对HL-60细胞中c-JUN及c-FOS在mRNA及蛋白水平的表达变化。结果:A3D8对HL-60细胞生长有抑制作用;A3D8可诱导细胞周期G_0/G_1期阻滞;A3D8可降低HL-60细胞c-JUN mRNA及蛋白表达水平,而对cFOS mRNA及蛋白表达无影响。结论:A3D8通过下调c-JUN转录及蛋白表达影响急性白血病细胞转录因子AP-1活性,抑制白血病细胞生长。

Objective：To explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells.Methods：After acute leukemia cell line HL-60 was treated by different concentrations of A3D8,the proliferation and cell cycle were detected by MTT and FCM respectively.The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively.Results：The proliferation of HL-60 was inhibited by A3D8.The A3D8 treatment increased the percentage of G0/G1 cells.The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8.The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group.Conclusions：A3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.