儿童急、慢性特发性血小板减少性紫癜HPA基因多态性分析

Analysis of HPA gene Polymorphisms in Children with Acute or Chronic ITP

目的:探讨广西地区儿童急、慢性特发性血小板减少性紫癜(ITP)的HPA-2和HPA-15等位基因频率分布情况及HPA-2和HPA-15基因多态性与儿童ITP发病的潜在联系。方法:收集2010年1月至2014年12月确诊为急性和慢性ITP的各46例患儿的临床资料,运用聚合酶链反应技术(PCR)结合直接测序方法,对急、慢性ITP两组各46例患儿及对照组48例健康儿童进行HPA-2和HPA-15基因分型。结果:3组之间HPA-2和HPA-15等位基因频率比较均有显著性差异(P<0.05),每2组比较分析显示,HPA-2和HPA-15等位基因频率在慢性ITP组与对照组间差异显著,有统计学意义(P<0.05),而急性ITP组与对照组及慢性ITP组与急性ITP组相比较差异均无统计学意义(P>0.05)。结论:HPA-2与HPA-15基因多态性与儿童慢性ITP发病有关,与儿童急性ITP发病无关。

Objective：To investigate the frequency distribution of human platelet antigen allele HPA-2 and HPA-15 among the pediatric patients with acute or chronic idiopathic thrombocytopenic purpura（ITP） in Chinese Guangxi area,and to explore the potential correlation of ITP with HPA-2 and HPA-15 gene polymorphisms.Methods：The clinical and laboratorial data of 46 children diagnosed as acute ITP and 46 children diagnosed as chronic ITP between January 2007 and December 2014 were collected.Genotyping of HPA-2 and HPA-15 in 92 ITP patients and 48 healthy controls was performed by using polymerase chain reaction（PCR） combined with direct sequencing.Results：The allele frequencies of HPA-2 and HPA-15 were significantly different among the acute,chronic and control groups;the allele frequencies were significantly different between the chronic ITP group and the control group（P〈0.0167）,while the difference was not statistically significant between the acute and chronic ITP groups as well as between the acute ITP and the control group（P 〉0.0167）.Conclusion：The gene polymorphism of HPA-2 and HPA-15 may correlate with the risk of chronic ITP,but may not correlate with acute ITP in children.