2型糖尿病患者糖原合成酶激酶3β的表达及活性

The protein expression and activity of glycogen synthase kinase 3β in the peripheral blood mononuclear cells of type 2 diabetic patients

目的探讨糖原合成酶激酶3β(GSK-3β)在2型糖尿病患者和健康者外周血单个核细胞中的蛋白表达及活性水平。方法运用酶联免疫吸附法(ELISA)检测30例2型糖尿病血糖未控制患者(实验1组)、30例2型糖尿病血糖控制患者(实验2组)和30例健康者(健康对照组)外周血单个有核细胞GSK-3β蛋白表达及活性水平,并分析其结果。结果2型糖尿病患者GSK-3β蛋白水平比健康对照组升高(P<0.05),实验1组GSK-3β活性水平比健康对照组和实验2组升高(P<0.05);实验2组GSK-3β蛋白水平比健康对照组升高(P<0.05)。实验1组男性GSK-3β活性水平比健康对照组男性升高(P<0.05)。结论 2型糖尿病患者GSK-3β蛋白高表达。经药物治疗后血糖控制者GSK-3β蛋白仍高表达,但活性水平明显下降。2型糖尿病血糖未控制男性患者GSK-3β活性水平明显增高。

Objective To investigate the clinical value of observing the differences of GSK-3βprotein expression and activity level in the peripheral blood mononuclear cells between type 2diabetes mellitus patients and healthy people.Methods Using ELISA to detect the GSK-3βprotein expression and activity levels of people who contained 30 patients with type 2diabetes whose blood glucose were not controlled（test group 1）,30 cases of patients with type 2diabetes whose blood glucose were controlled（test group2）and 30 cases of healthy human and all the data were analyzed.Results The GSK-3βprotein content of Type 2diabetes mellitus group was higher than the control group（P〈0.05）,the activity level of GSK-3βin test group 1was higher than the control group and test group（P〈0.05）.The GSK-3βprotein content of test group 2 was higher than the control group（P〈0.05）.The male GSK-3βactivity level in the test group 1was higher than the men′s in the control group（P〈0.05）.Conclusion The GSK-3βprotein content has a high expression in the type 2diabetes mellitus patients.After drug treatment,the patients,who have controlled the blood glucose effectively,still showes high expression of the GSK-3βprotein content,but the activity levels showes a significant decline.In male patients with type 2diabetes blood sugar in the control group of GSK-3βshowes significantly higher activity level.