银耳五加参胶囊对小鼠免疫功能的影响

Effects of Tremella Acanthopanax capsule on mice immune functions

目的研究银耳五加参胶囊对小鼠免疫功能的影响,以期为银耳和五加参的开发利用提供实验支持。方法经口灌胃分别给予健康雌性清洁级ICR小鼠纯净水(对照组,5批,每批10只)、银耳五加参胶囊87.5 mg/kg(低剂量组,5批,每批10只)、175 mg/kg(中剂量组,5批,每批10只)、525 mg/kg(高剂量组,5批,每批10只),每日1次,连续30 d后,分别进行小鼠脾淋巴细胞转化试验和自然杀伤(NK)细胞活性测定、二硝基氟苯(DNFB)诱导小鼠迟发性变态反应(DTH)试验、抗体生成细胞检测和血清溶血素测定、小鼠碳廓清试验、小鼠腹腔巨噬细胞吞噬鸡红细胞试验,测定小鼠免疫功能。结果低、中、高剂量组小鼠的巨噬细胞吞噬鸡红细胞的能力均高于对照组(P<0.05,P<0.01)。高剂量组小鼠的NK细胞活性和DNFB诱导的小鼠DTH能力均高于对照组(P<均0.01)。在受试剂量下银耳五加参胶囊对小鼠刀豆蛋白A诱导的小鼠脾淋巴细胞转化能力、抗体生成细胞数、半数溶血素值(HC50)、小鼠碳廓清能力均无影响(P均>0.05)。结论银耳五加参胶囊有增强正常小鼠免疫力的作用。

Objective To study effects of Tremella Acanthopanax capsule on mice immune functions to provide experimental support for the exploitation and utilization of Tremella and Acanthopanax. Methods A total of 200 female healthy clean grade ICR mice were divided into four groups（ 5 batch,10 rats in each batch for each group） ： control group and three Tremella Acanthopanx capsule groups [low-dose group（ 87. 5 mg / kg）,medium-dose group（ 175 mg / kg）,high-dose group（ 525 mg/kg） ],and the purified water,Tremella Acanthopanax capsule of low-,medium-,high-dose were respectively given by gavage administration per oral（ one time a day,for 30 days）. Then,mouse spleen lymphocyte transformation test,natural killer（ NK） cell activity assay,dinitrofluorobenzene（ DNFB）-inducing mouse delayed type hypersensitivity（ DTH） test,antibody-producing cell detection,determination of serum hemolysin,mouse carbon clearance test and mouse peritoneal macrophage phagocytosis of chicken red blood cell test were used to detect mice＇ s immune function. Results The capacities of macrophage phagocytosis of chicken red blood cells in group low-,medium-,high-dose were all significantly higher than those in control group（ P〈0. 05,P〈0. 01）. The NK cell activity and the capacity of DNFB-inducing mouse DTH in highdose group were all significantly higher than those in control group（ all P〈0. 01）. Under the trial dose,Tremella Acanthopanax capsule had no effects on concanavalin A（ Con A）-inducing mouse spleen lymphocyte transformation ability,antibody-producing cell number,half hemolytic value（ HC50） and mouse carbon clearance ability（ all P〈0. 05）. Conclusion Tremella Acanthopanax capsule can enhance the immune functions of normal mice.