水通道蛋白在糖尿病大鼠阴道组织的表达

Expression of aquaporins in vagina of diabetic rat

目的:研究AQP 0、5、6、10、11及12在糖尿病大鼠阴道组织的表达。方法:将糖尿病大鼠随机分为12、16周组并将正常大鼠随机分为12、16周组(n=6)。分别测各组大鼠阴道润滑及AQPs在阴道组织的表达情况。结果:本实验中12、16周龄糖尿病大鼠血糖水平分别较12、16周对照组大鼠血糖水平显著升高(P<0.05),12及16周龄糖尿病大鼠阴道润滑性(2.12±1.41;2.64±0.88)分别较12及16周对照大鼠血糖阴道润滑性(4.21±0.86;4.41±0.77)显著降低(P<0.05),糖尿病大鼠阴道组织中AQPs的表达均低于相应对照大鼠阴道组织中AQPs的表达(P<0.05)。结论:糖尿病大鼠阴道组织AQPs的表达下降可能导致其阴道润滑性下降。

Objective： The aim is to investigate the expression of AQP 0, 5, 6, 10, 11 and 12 in the vaginal tissue of diabetes mellitus rats. Methods： Female Sprague-Dawley rats （n = 24） were randomly divided into 4 of AQP 0, 5, 6, 10, 11 and 12 in vaginal tissue of rats were determined. Results： The levels of blood glucose were significantly increased in 12-week-old diabetic rats and 16-week-old diabetic rats compared to their age matched counterparts respectively（P 〈 0.05）, vaginal lubrication was significantly lower in 12-week-old diabetic rats （2.12 ± 1.41 ） and 16-week-old diabetic rats （2.64 ±0.88） than their age matched counterparts, （4.21 ± 0.86） and （4.41 ± 0.77） （P 〈 0.05）, respectively; and the expression of AQP 0, 5, 6, 10, 11 and 12 in vaginal tissue were significantly lower in 12-week-old diabetic rats and 16-week-old diabetic rats than their age matched counterparts respectively （P 〈 0.05）, as determined by immunohistochemistry and western blot, respectively. Conclusions： Decreased vaginal lubrication in diabetic rats may be related to the decreased expression of AQPs in vaginal tissue.