成纤维细胞生长因子受体家族在头颈鳞癌细胞中的表达及定位

Expression and localization of FGFR family in squamous cell carcinoma of head and neck

目的:探讨成纤维细胞生长因子受体在头颈鳞癌细胞中的表达及其定位。方法:提取10种头颈鳞癌细胞系蛋白,采用Western印迹技术检测FGFR家族成员的表达;利用免疫荧光技术检测FGFR各型在SCC25和HN6细胞中的表达位置。应用Image J软件测量免疫印迹条带灰度值,Graph Pad Prism 5.01对灰度值结果进行统计学分析。结果:在10株头颈鳞癌细胞细中,6株表达FGFR1。FGFR2、3、4均在10株头颈鳞癌细胞细中表达。FGFR1、2、4表达于细胞核及细胞质,FGFR3仅表达于细胞质。结论:FGFR在头颈鳞癌细胞细中共表达,可能与头颈鳞癌的发生、发展有关;FGFR家族不同亚型在头颈鳞癌细胞的表达定位存在差异。

PURPOSE： To investigate the expression and localization of FGFR family in squamous cell carcinoma of head and neck （SCCHN） cell lines. METHODS： Total protein was extracted from 10 SCCHN cell lines and the expression of FGFR was detected by Western blot. The localization of FGFR was further demonstrated by immunofluorescence staining in SCC25 and HN4 cell lines. Gray value was measured by Image J. GraphPad Prism 5.01 software package was used for data processing and analysis. RESULTS： FGFR1 expression was detected in 6/10 cell lines and FGFR2, 3, 4 was detectable in all cell lines. The expression of FGFR1, 2, 4 was mainly in the nucleus and cytoplasm while FGFR3 was predominantly localized in cytoplasm. CONCLUSIONS： FGFR shows co-expression in SCCHN cell lines, which may be associated with the tumorigenesis and development of SCCHN.