MMPs抑制剂对湿性黏结微渗漏的影响

Effects of MMPs inhibitors on microleakage with wet bonding technique

目的：研究牙本质湿性黏结时,基质金属蛋白酶（matrix metalloproteinases,MMPs）抑制剂对黏结界面微渗漏的影响。方法：收集人离体前磨牙27颗,平均分成3组。将牙体颈部统一制备V类窝洞,酸蚀后分别用蒸馏水、2%氯己定（chlorhexidine,CHX）和2%米诺环素（monocycline,MI）在窝洞内均匀涂抹60 s,然后黏结、树脂填充、固化。用10%次氯酸钠（sodium hypochlorite,NaClO）溶液老化1 h,再涂布2层指甲油,50wt%氨化硝酸银（ammoniated silver nitrate,ASN）和显影液处理各组牙。最后将牙切成1~2 mm的牙片,应用体式显微镜和扫描电镜观察充填树脂与牙本质之间银颗粒渗入情况,采用SPSS 16.0软件包对各组微渗漏程度进行Wilcoxon秩和检验。结果：各组充填树脂周边均有不同程度的微渗漏情况。经Wilcoxon秩和检验,CHX组与对照组、MI组与对照组的差异有显著性;CHX组与MI组相比,差异无显著性。结论：牙体湿性黏结时,MMPs抑制剂能减轻黏结界面微渗漏的程度。

PURPOSE： To observe the effects of matrix metalloproteinases （MMPs） inhibitors on microleakage with wet bonding technique. METHODS： Twenty-seven premolars were randomly assigned to 3 groups. Class V cavities were prepared in the neck of the teeth and treated with distilled water, 2% chlorhexidine （CHX） or 2% monocyeline （MI） for 60 seconds. Resin was used to restore the prepared cavities following application of luting agent. Samples in each group were soaked for 1 h with 10% NaC10 and then 2 layers of nail polish were applied, followed by treatment with 50wt% ammoniated silver nitrate （ASN）, and developing solution in turn. Each tooth was sectioned longitudinally to 1~2 mm slices and examined with stereo microscope and scanning electron microscope （SEM）. The data were analyzed with SPSS 16.0 software package for Wilcoxon rank sum test. RESULTS： There was varied microleakage at the margin. The difference was significant between group CHX and control group, between group MI and control group; However, there was no significant difference between group CHX and group MI. CONCLUSIONS： MMPs inhibitor can reduce microleakage with wet bonding technique.