吉西他滨对人非小细胞肺癌HCC827活性和凋亡的影响

Effect of Gemcitabine on viability and apoptosis of non-small cell lung cancer HCC827 in vitro

目的：研究吉西他滨对人非小细胞肺癌HCC827细胞活性以及凋亡的影响,以及与Bcl-2表达的关系。方法：采用cell counting kit-8法、细胞核Hochest33258染色法、流式细胞仪Annexin V-FITC/PI双染法等多种方法,体外研究吉西他滨对人非小细胞肺癌HCC827的活性、凋亡和细胞周期的影响;采用Western blot法,观察药物作用后细胞中Bcl-2蛋白表达的变化。结果：吉西他滨（0.1～1 000 ng/ml）对人非小细胞肺癌HCC827活性具有抑制作用,并有促凋亡效应。此外,可以将细胞阻滞在S期。在吉西他滨诱导的细胞凋亡过程中,凋亡因子Bcl-2蛋白的表达下调。结论：吉西他滨可以抑制人非小细胞肺癌HCC827细胞的增殖,并诱导细胞凋亡,S期周期阻滞,其诱导的凋亡可能是其杀死肿瘤细胞的主要机制之一,此外,凋亡与相关基因Bcl-2蛋白的表达具有一定关系。

Objective： To observe the effect of Gemcitabine（ GEM） on the viability and apoptosis of non-small cell lung cancer HCC827 in vitro. Methods： The cell viability,apoptosis and cell cycle of HCC827 cells induced by Gemcitabine were detected with cell counting kit-8 assay（ CCK-8）,Annexin V-FITC / PI staining and flow cytometry. The expression of Bcl-2 protein of cells treated with GEM was examined by Western blot assay. Results： There was significant inhibition effect on HCC827 cells treated with 0. 1- 1 000 ng / ml of GEM,which can promote the occurrence of HCC827 cell apoptosis and arrest cell in the S phrase. The apoptosis induced by GEM was accompanied with the down regulation of Bcl-2 protein. Conclusion： GEM can inhibit the cell viability and induce the HCC827 cell apoptosis and S phrase arrest. Its cell dead type was apoptosis,which was related with the expression of Bcl-2 protein.