Foxp3对肺癌细胞增殖及成瘤的影响

Enforced expression Foxp3 enhances proliferation and tumorigeneity of lung cancer cell

目的:研究转录因子Foxp3在肺癌细胞中的高表达对肺癌细胞增殖及成瘤的影响。方法:利用脂质体转染法建立稳定高表达Foxp3的NCIH-h Foxp3肺癌细胞株。MTT法观测NCIH-h Foxp3及对照细胞的增殖。ELISA法检测NCIHh Foxp3及对照细胞IL-8、IL-10的分泌。建立移植瘤小鼠模型,观察成瘤情况。结果:成功建立高表达Foxp3的NCIH-h Foxp3肺癌细胞株;与阴性对照相比,NCIH-h Foxp3细胞增殖减慢,但成瘤能力强。NCIH-h Foxp3细胞培养上清IL-8表达量减低,IL-10表达量升高。结论:高表达Foxp3的肺癌细胞可能通过表达细胞因子改变局部生长的微环境,逃逸免疫监视,而促进肿瘤细胞的成瘤和发展。

Objective： To determine the effects of enforced expression of Foxp3 in lung cancer cell with regards to proliferation and tumorgeneity. Methods： A stable subline NCIH-h Foxp3 was established by liopfectamin-mediated pc DNA plasmid transfection carrying exogenous h Foxp3. The growth curve and secrection of IL-8 and IL-10 of NCIH-h Foxp3 were evaluated using MTT and ELISA,respectively. The in vivo tumorigeneity was assessed as well by inoculation of NCIH-h Foxp3 subcutaneously in nude mice. Results： Lung cancer cell NCIH-h Foxp3 with enforced expression of Foxp3 proliferated slowly but exihited increased in vivo tumorgeneity compared with corresponding control subline. In addition,increased expression of h Foxp3 in NCIH-h Foxp3 augmented secretion and attenuated secretion of IL-8 and IL-10,respectively. Conclusion： Increased expression of Foxp3 may promote progression of lung cancer cell by change of cellular microenvironment and evasion of immune surveillance.