西马特罗分子印迹荧光检测试纸条的研制

A Novel Molecularly Imprinted Fluorescence Test Strip for Detection of Cimaterol

将分子印迹技术良好的选择性与试纸条快速检测的特点相结合,研制了一种快速检测西马特罗的分子印迹试纸条。以西马特罗为模板分子,化学聚合法制备西马特罗分子印迹聚合物,将其附着在硝酸纤维素膜上。洗脱模板分子后,裁剪压制得到分子印迹试纸条。试纸条识别样品中西马特罗后,滴加荧光物质曙红Y,根据反应区内西马特罗与曙红Y反应后荧光猝灭程度对西马特罗进行分析。研究结果表明,在0.01~100μg/m L浓度范围内,荧光猝灭程度随着西马特罗浓度的增加而增大,最低检测浓度为0.01μg/m L。此试纸条制备简单,操作简便,可用于现场检测。将其用于猪肉和饲料样品中西马特罗的快速测定,结果令人满意。

A novel test strip for rapid detection of cimaterol was prepared by combining highly specific selectivity of molecularly imprinted technology with convenience and rapid response performance of test strip.The cimaterol molecularly imprinted polymers（ MIPs） were prepared through chemical polymerization method,and the nitrocellulose membrane was immersed into MIPs solution. After that,the template molecules were eluted and then a clipping step was conducted for obtaining the test strip. After re-adsorption of cimaterol,fluorescent dye Eosin Y was added onto the surface reaction zone on the strip. Qualitative and quantitative analysis could be realized via determining fluorescence quenching in the reaction zone. The experimental results showed that the quenching of fluorescence had a good linear relationship with the cimaterol concentration ranging from 0. 01 to 100 μg / m L with a limit of detection of 0. 01 μg / m L. The molecular imprinting test strips provided a convenient way in practical application especially for the quick detection of pork and fodder sample on site.