沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响

Effect of Interferon Inducible Protein 16 on Apoptosis of Human Aortic Outer Membrane Fibroblasts

目的探究沉默干扰素诱导蛋白16(IFI16)对人主动脉外膜成纤维细胞(HAAFs)凋亡的影响。方法检测HAAFs凋亡、IFI16、细胞外调节蛋白激酶1/2(ERK1/2)及磷酸化细胞外调节蛋白激酶1/2(p-ERK1/2)蛋白表达。结果基因沉默组IFI16低于另两组(P<0.05),基因沉默组ERK1/2及p-ERK1/2蛋白表达高于另两组(P<0.05),阴性对照组及空白组HAAFs凋亡率、IFI16、ERK1/2及p-ERK1/2蛋白表达差异无统计学意义(P>0.05)。结论沉默IFI16表达能够降低HAAFs凋亡率。

Objective To explore the effect of silencing interferon inducible protein 16（IFI16）on the apoptosis of human aortic outer membrane fibroblasts（HAAFs）. Methods Detection of HAAFs apoptosis,IFI16 and extracellular regulated protein kinase 1/2（ERK1/2）and phosphorylated extracellular regulated protein kinase 1/2（p-ERK1/2）protein expression. Results Gene silencing of group IFI16 was lower than that of the other two groups（P 〈0.05）,gene silencing and p-ERK1/2 protein expression in ERK1/2 group was significantly higher than that of the other two groups（P 〈0.05）,negative control group and blank control group HAAFs,IFI16,ERK1/2 apoptosis and p-ERK1/2 protein expression differences were not statistically significant（P 〉0.05）. Conclusion Silencing IFI16 expression can reduce the apoptosis rate of HAAFs.