甲基莲心碱体外抑制人肝癌细胞增殖和侵袭的作用机制研究

Effect of neferine on proliferation and invasion of human hepatocellular carcinoma cell line HepG2 and Bel-7402

目的探讨甲基莲心碱(neferine,Nef)对人肝癌HepG2和Bel-7402细胞侵袭的影响和作用机制。方法人肝癌HepG2和Bel-7402细胞体外培养,经不同浓度的甲基莲心碱处理后,CCK-8法检测细胞的增殖,Transwell细胞体外侵袭实验观察Nef对细胞侵袭能力的影响;免疫印迹检测Rho相关蛋白的表达。结果 CCK-8结果显示,与对照组比较,甲基莲心碱干预组抑制人肝癌HepG2和Bel-7402细胞增殖,并呈剂量-效应关系(P<0.05);Transwell细胞体外侵袭实验显示,3μmol·L^(-1)甲基莲心碱明显抑制HepG2和Bel-7402细胞的侵袭;免疫印迹结果显示,3μmol·L^(-1)甲基莲心碱作用HepG2和Bel-7402细胞12 h后,RhoA、RhoC和ROCK表达明显降低。结论甲基莲心碱可体外抑制HepG2和Bel-7402细胞的增殖和侵袭,其抑制肝癌细胞的侵袭可能与抑制RhoA、RhoC和ROCK蛋白的表达有关。

Aim To investigate the effect of neferine on proliferation and invasion of human hepatocellular carcinoma. Methods HepG2 and Bel-7402 cells were cultured in vitro with different concentrations of neferine,then cell proliferation was observed by CCK-8 assay; cell invasion was observed by transwell invasion assay; the protein expression of RhoA, RhoC and ROCK was detected by Western blot. Results CCK-8results showed that neferine could significantly inhibit cell proliferation in a dose-dependent manner compared with the control group. Transwell invasion assay showed that cell invasion was significantly decreased with neferine 3 μmol · L^(-1). Western blot results showed that RhoA,RhoC and ROCK protein expression was decreased when neferine was co-incubated with hepatocellular carcinoma cells. Conclusion Neferine can inhibit proliferation and invasion of HepG2 and Bel-7402 cells,which is mediated mainly by the inhibition of RhoA,RhoC and ROCK protein expression.