单核细胞趋化蛋白-1基因多态性与浙江局部地区汉族人群2型糖尿病的相关性研究

Association of monocyte chemoattractant protein- 1 gene polymorphism with risk of type 2 diabetes mellitus in Han population in Zhejiang province

目的探讨单核细胞趋化蛋白-1（MCP-1）基因多态性与浙江局部地区汉族人群2型糖尿病（T2DM）的相关性.方法采用病例对照研究,选取浙江局部地区汉族人群中T2DM 患者208 例（T2DM 组）和健康体检者209 例（对照组）,采用聚合酶链反应- 限制性片段长度多态性方法检测受试者CCL2 rs1024611（-2518A/G）的基因型,并用DNA 测序法鉴定部分结果.结果CCL2 rs1024611 基因多态性与T2DM 发生相关,T2DM 组和对照组G 基因频率差异有统计学意义（P〈0.01）,T2DM 组和对照组GG基因型频率差异有统计学意义（P〈0.01）,多因素logistic回归分析显示,GG基因型频率可显著降低T2DM 风险（校正后OR=0.201,95%CI0.077-0.526,P〈0.01）.结论CCL2 rs1024611 基因多态性与T2DM发生相关,其中GG 基因型可能是T2DM 的保护性因素,G等位基因可能降低汉族浙江局部地区汉族人群T2DM的风险.

Objective To investigate the association of monocyte chemoattractant protein-1 （MCP-1） gene rs1024611（-2518A/G） polymorphisms with risk of type 2 diabetes mellitus （T2DM） in Han population of Zhejiang province. Methods Atotal of 208 patients with T2DM and 209 healthy control subjects in Han population of Zhejiang province were enrolled in thisstudy. Genotyping was performed by polymerase chain reaction-based restriction fragment length polymorphism andsubsequently confirmed by DNA sequencing. Odds ratio（OR） and 95% confidence intervals（CI） were calculated for the risk ofgenotypes and alleles. Results MCP-1 gene polymorphism rs1024611 （-2518A/G） was conformed to the Hardy-Weinbergequilibrium. The statistical differences in the G allele （43.7% vs.55.7%, P〈0.01） and GG genotype （19.2% vs.33.5%, P〈0.01）were found between T2DM patients and controls. The GG genotype was associated with a decreased risk of T2DM（OR=0.201,95% CI 0.077-0.526,P〈0.01）. Conclusion The MCP-1 gene rs1024611（-2518A/G） may be associated with T2DM susceptibilityin Han population of Zhejiang province; GG genotype may be the protect factor for T2DM and G allele may reduce therisk of T2DM.