急性低氧暴露下大鼠脑组织的病理学改变及脑IL-6、TNF-α的表达变化

The pathological alteration of rats' brain under acute hypoxia exposure and the expression change of IL-6,TNF-α

目的探讨在模拟高原环境中,急性低氧暴露大鼠脑组织的病理学改变及IL-6、TNF-α的表达变化。方法SD大鼠50只雌雄各半,实验分5组。分别是常压对照组,急性低压低氧暴露1 d组、2 d组、3 d组以及7 d组,暴露后测定大鼠脑干湿重比,脑组织中TNF-α、IL-6含量及病理学改变。结果与正常对照组相比,暴露1 d组、2 d组、3 d组大鼠脑干湿重比、脑组织中IL-6、TNF-α升高(P<0.05)。暴露7 d组脑组织含水量、脑组织中IL-6、TNF-α均显著低于暴露3 d组(P<0.05)。病理结果显示正常对照组脑组织细胞形态结构及组织间隙正常,暴露3 d组脑组织分子层、锥体细胞周围、血管周围、脉络丛以及脑膜下水肿,暴露7 d组脑组织分子层水肿较3 d组减轻。结论在模拟海拔5 km低压低氧环境中,SD大鼠的脑组织的病理学改变以及干湿重比、TNF-α、IL-6因子含量均在3 d时显著改变和升高,但随着暴露时间的延长,损伤程度有所降低,这和平原人群进入高原后所产生的低氧习服机制相同。故该研究所选择观察的SD大鼠脑组织的形态学改变以及检测脑组织中TNF-α、IL-6含量改变可作为急性高原病模型是否成模的判定指标。

Objective To discuss under the simulated plateau environment, the rats＇ brain exposed to the hypobaric hypoxia, the pathological alternation of the rats＇ brain exposed to the hypobaric hypoxia, and the expression change of IL-6, TNF-α, Method Half male and half female, 50 SD rats, and the experiment is divided into five groups. They are atmospheric pressure in the control group, hypobaric hypoxia group, exposure 1 d group, 2 d group, 3 d group and 7 d group. Test the rats＇ brain dry to wet weight ratio. The pathological alternation and the content of the TNF-α and IL-6. Result Compare the normal contrast group. In the contrast, exposure 1 d group, 2 d group, 3 d group dry to wet weight ratio, the brain tissue of TNF-a and IL-6 go up （p 〈 0. 05）. Exposure 7d group： water content of brain tissue and the TNF-α and IL-6 brain tissue both markedly under the exposure 3 d group （p 〈 0.05）. Pathological analysis showed that normal contrast brain tissue＇ s cellular forms and structure and inter-cellular space all are normal. Exposure 3 d group brain tissue molecular layer centrum cells, blood vessel choroid plexus, meninx edema, exposure 7 d group brain tissue molecular layer drop compared with 3 d group. Conclusion Under the environment of simulation plateau, SD rats＇ pathological alternation and dry to wet weight ratio. TNF-α and IL-6 factor content grow significantly at 3d group. But with the extension of exposure time, the injury degree decrease comparatively. This is same with plain people come into plateau, producing hypoxia mechanism. Therefore, research institution chose SD rats brain tissue＇ s morphological changes and test the content alternation of TNF-α , IL-6 as the criterion of the hypobaric plateau model whether it will become model or not.