正常人体甲状腺体表傅里叶红外光谱图的特征分析

Characteristics Analysis of the Thyroid Gland Surface of Normal Human with Fourier Transform Infrared(FTIR)Spectra

应用傅里叶变换红外光谱仪(FTIR)并联合衰减全反射(ATR)探头经体表方式测定120名健康受试者的双侧甲状腺,由此获得240张正常人体甲状腺体表傅里叶红外光谱图;通过分析比较正常人体左右两侧甲状腺体表红外光谱12个谱带的峰位、相对峰强及半高宽等35个FTIR光谱参数以探讨双侧甲状腺体表红外光谱有无差异;同时明确甲状腺体表红外光谱中蛋白质、脂质、核酸和糖类等生物化学成分相应特征吸收峰的变异特征。结果表明:正常人体左右两侧甲状腺体表红外光谱图大体趋于一致,仅F1 640P2 920P1 040和I2 920/I1 460,4个光谱参数差别存在统计学意义;并计算得到一系列正常人体甲状腺体表傅里叶红外光谱特征谱带光谱参数的90%正常参考值范围及绘制出能充分代表正常人体甲状腺基本特征的标准平均红外光谱图,进一步为傅里叶变换红外光谱应用于临床甲状腺疾病诊断提供参考。

In order to obtain 240 cases of the surface Fourier transform infrared（FTIR）spectra of normal human thyroid gland,120 healthy volunteers were enrolled in this study and scanned by using two mid-infrared optical fibers equipped with an attenuated total reflectance（ATR）to probe through the skin of two lobes of thyroid gland.Then whether there were differences between the surface FTIR spectra of the right lobe and that of the left part of the normal human thyroid gland was explored by comparing the 35 variables of 12 bands,including peak positions,relative intensity ratios and full width at half maximum of FTIR spectrums.Also the spectra characteristics of unique absorbent bands that represent changes of structure and quantity of variance biochemical substances such as nucleic acids,proteins and carbohydrates were illustrated.Results indicated that the surface FTIR spectra of the right part and that of the left part of the normal human thyroid gland were generally coincided,with statistically significant differences in four spectrum parameters,includingF1 640,P2 920,P1 040 and I2 920/I1 460.A series of data about the90% normal reference range of the unique spectrum parameters in the surface FTIR spectra of normal human thyroid gland was gained,and a standard average infrared spectrogram which can fully represent the basic features of normal human thyroid gland was plotted.The conclusions above could be used as a reference for clinical diagnosis of thyroid diseases with FTIR spectroscopy.