基于HPLC-RI与FAAS对云南春蜂蜜理化性质的研究

Study Physicochemical Characteristics of Spring Honeys from Yunnan with the Application of HPLC-RI and FAAS

云南地区因其得天独厚的自然条件,具有丰富的蜜源植物和矿产资源,产出了多种特色蜂蜜。对该地区的三种特色春蜂蜜(苕子蜜、橡胶蜜和石榴蜜)进行了理化性质的研究,结果表明三种蜂蜜在水分含量、电导率和粘度上均存在显著性差异。利用高效液相色谱仪-示差折光检测器(HPLC-RI)测定三种蜂蜜中的主要糖类,结果显示石榴蜜中的葡萄糖含量最高[35.62g·(100g)-1],橡胶蜜中的果糖含量最高[41.03g·(100g)-1]。利用原子吸收光谱仪(FAAS)对三种蜂蜜中的13种矿质元素进行测定,结果显示橡胶蜜中的矿质元素总含量最高(437.34mg·kg-1),石榴蜜中的最少(167.24mg·kg-1)。将矿质元素作为变量进行主成分分析,可将三种蜂蜜相互分开。Cu,Zn和Na可作为苕子蜜的分辨标记,Mg,K,Ca,As和Cd可作为橡胶蜜的分辨标记,Fe,Mn,Ni和Cr可作为石榴蜜的分辨标记。本研究的开展可补充云南特色蜂蜜的研究,为其开发利用提供理论依据。

Many special honeys are produced in Yunnan province due to abundant nectar plants and minerals resources provided by the unique natural environment in this area.In this work,the physicochemical property of three honeys（Viciacracca honey,Hevea brasiliensis honey and Punica granatum honey）from Yunnan was studied.The results showed that in different honeys the moisture content,electrical conductivity and dynamic viscosity were different.The sugar contents of each honey were determined with HPLC-RI.The results showed that P.granatumhoney had the most abundant glucose[35.62g·（100g）-1],and H.brasiliensis honey had the most abundant fructose[41.03g·（100g）-1].Thirteen different mineral elements in three honey species were determined with FAAS.It was found that the mineral level was from 167.24mg·kg-1 in P.granatum honey to437.34mg·kg-1 in H.brasiliensis.Based on the mineral content the three honey species were classified following the principal component analysis（PCA）method.The result showed that Cu,Zn and Na could act as the elemental markers for V.cracca honey,while Mg,K,Ca,As and Cd act as the elemental markers for H.brasiliensis honey,and Fe,Mn,Ni,and Cr act as the elemental markers for P.granatumhoney.This study reported the physicochemical property of three special Yunnan honeys,which could help the further study and utilization of these honeys.