颅内出血患者血清IL-6、TNF-α变化在法医临床鉴定中的意义

Forensic clinical significance of detecting the serum levels of IL-6 and TNF-ain patients with intracranial haemorrhage

目的探讨颅内出血患者血清IL-6、TNF-α的水平变化及在法医学的临床意义。方法采用酶联免疫吸附法(ELISA)对颅内出血患者住院后不同时间段血清IL-6、TNF-α水平进行检测,并与正常对照组的健康受试者进行比较分析。结果研究组重型与轻型患者后第1、3、5、7天血清IL-6、TNF-α水平较正常对照组明显增加,重型患者入院第5、7天的血清IL-6、TNF-α较轻型患者明显增加,差异有统计学意义(P<0.05);根据患者最后结果(死亡组和存活组),发现死亡组随着时间延长,在第5、7天时IL-6、TNF-α水平显著高于存活组(P<0.05)。此外,血清IL-6和TNF-α水平呈正性显著相关(r=0.721,P<0.05)。结论检测颅内出血患者血清IL-6、TNF-α水平对观察其病情变化有重要意义,可作为法医学鉴定的辅助手段。

Objective To explore the clinical significance of detecting serum levels of IL-6 and TNF-α in patients with intracranial hemorrhage. Methods The serum levels of IL-6 and TNF-α in different period were detected in patients with intracranial hemorrhage, by enzyme-linked immunosorbent assays （ELISA）, and compared with those of healthy subjects （the control group）. Results The serum levels of IL-6, TNF-α in the severe and slight patients of study group on 1st, 3rd, 5th and 7th were significantly higher than those in the control group （all with P〈0.05）. The serum levels of IL-6, TNF-a in the severe patients of study group were significantly higher than those in slight patients of study group （all with P〈0.05） on 5th and 7th. The serum levels of IL-6 and TNF-α in dead cases on 5th, 7th days admission were significantly higher than those in survival cases （P〈0.05）. The serum levels of IL-6 was positively correlated with TNF-α （r=0.721, P〈0.05）. Conclusion The detection of dynamically serum levels of IL-6 and TNF-α is of great clinical value for assessing the disease development therapeutic efficacy and prognosis of brain injury patient with intracranial hemorrhage.