摘要
在各单因素实验基础上,以总黄酮提取率为指标,通过Box-Behnken响应面设计考察乙醇浓度、料液比、提取时间、提取次数对地枇杷总黄酮提取工艺的影响,优化贵州野生地枇杷总黄酮提取工艺。结果表明:地枇杷总黄酮的乙醇回流最佳提取工艺条件的酒精浓度为70%,液固比1∶15,提取时间为30 min,提取次数为3次,地枇杷总黄酮提取率为5.09%。按最优提取条件进行3次验证实验,最终地枇杷总黄酮平均提取率5.13%。本文利用乙醇回流提取地枇杷总黄酮工艺操作简便,稳定可行。
In this research we tested the effects of ethanol concentration,solid-liquid ratio,time and period of extraction on extraction rateof total flavonoids from F.tikoua by Box-Behnken design to optimize extracting technology based on single factor test. The results showed that,the optimum ethanol reflux extraction conditions of total flavonoids in F.tikouawas as follow： extracting 3 times with the alcohol concentration of 70%,liquid-solid ratio of 15,30 min each time.The yield of total flavonoids was 5.09%. The optimum extraction conditions wasverifiedforthree times,and the average yield of total flavonoids was 5.13%. Extracting total flavonoids from wild F.tikoua by ethanol reflux process is a simple,stable and feasible method.
作者
陈琳
俸婷婷
游婵
李超
CHEN Lin FENG Ting-ting YOU Chan LI Chao(School of Pharmaceutical Sciences, Guizhou University, Guiyang, Guizhou 550025, China Me- dicinal and Edible Plant Resources Research and Development Center of Guizhou, Guiyang, Guizhou 550025, China Medicinal and Edible Plant Resources Application and Development Engineer Labora- tory of Guizhou,Guiyang, Guizhou 550025, China)
出处
《山地农业生物学报》
2016年第4期83-87,共5页
Journal of Mountain Agriculture and Biology
基金
贵州省科学技术基金项目(黔科合J字[2012]2173号)
贵州省科技合作计划项目(黔科合LH字[2015]7666号)
贵州省药食同源资源研究开发科技创新人才团队(黔科合人才团队(2015)4010号)
贵州省药食同源植物资源开发工程技术研究中心(黔科合G字(2015)4001)
贵州省药食两用资源应用开发工程实验室(黔发改投资[2015]542号)
贵州省中药民族药制药工程专业学位研究生工作站(黔教研合JYSZ字[2014]002)
