CRMS诱导水稻雄性不育的研究——Ⅲ.CRMS对水稻花药游离脯氨酸含量的影响及其与花药败育的关系

The Male Sterility in Rice Plant Induced by CRMS Ⅲ. Effect of CRMS on free proline content in anthers of rice and its possible relation to abortion of anther

利用~3H-脯氨酸、~3H-谷氨酸示踪分析方法,研究了CRMS诱导水稻雄性不育的作用机理。试验结果表明,CRMS阻碍剑叶向花药运输脯氨酸,抑制花药本身合成脯氨酸能力,并降低脯氨酸合成醇不溶性大分子物质的结合率,引起花药营养匮乏,导致花药败育。

The feeding experiment with ~3H-proline and infiltration one with ~3H-glutamic acid into an- thers were carried out to investige the effects of CRMS on proline transport from flag leaf into anthers. and the transformability of anthers from glutamic acid into proline and the possible cause of anther abor- tion. The results were obtained as follows: (1) There was significantly difference in the content of free proline in anthers between CK and CRMS treated plant at miosis of pollen mother cell. (2) The free proline in anthers mainly came from the vegetative parts, and this transporting process as an active one, could be inhibited by CRMS.The anther themselves can also transform glutamic acid into proline. These might result in the anther proline deficiency and its abortion.