补肺平喘汤对支气管哮喘大鼠Th1/Th2失衡及JAK/STAT信号通路影响

Bufei Pingchuan decoction on Th1/Th2 cytokine imbalance and JAK/STAT signaling pathway in asthmatic rats

目的观察补肺平喘汤对支气管哮喘大鼠Th1/Th2细胞因子失衡及JAK/STAT信号通路影响。方法将50只健康大鼠随机分为正常组,模型组,中药低剂量组,中药中剂量组,中药高剂量组5组,每组10只。除正常组外,其余各组给予卵白蛋白和氢氧化铝免疫反应制备支气管哮喘大鼠模型。造模4周后,中药低、中、高剂量组分别按照生药量10.8,21.6,32.4 g/kg进行灌胃给药,正常组和模型组给予等量生理盐水灌胃,灌胃4周后,分离大鼠血清和肺组织,Elisa法检测大鼠血清IFN-γ、IL-4水平变化,RT-PCR法检测大鼠肺组织JAK/STAT信号通路变化。结果与正常组比较,模型组大鼠血清IL-4水平显著升高(P<0.01),而IFN-γ呈显著下降趋势(P<0.01),IL-4/IFN-γ提示免疫调节显著向Th2水平失衡,肺组织JAK1、STAT6 mRNA水平明显升高;与模型组比较,中药中、高剂量组大鼠血清IL-4水平显著降低(P<0.05,P<0.01),IFN-γ水平显著升高(P<0.05),免疫调节明显向Th1方向调节(P<0.01),肺组织JAK1、STAT6 mRNA水平明显降低(P<0.05,P<0.01)。结论补肺平喘汤能有效改善支气管哮喘大鼠Th1/Th2细胞因子失衡,降低JAK1、STAT6 mRNA表达,调节Th1/Th2细胞因子平衡。

Objective To observe The influences of Bufei Pingchuan decoction on the Th1/Th2 cytokine imbalance and JAK/STAT signaling pathway in the asthmatic rats. Methods The 50 rats were divided randomly into Normal Group,Model Group,Low-dose Chinese herb Group,Medium-dose Chinese herb Group,High-dose Chinese herb Group,10 rats in each group. Except the normal group,the rest of the group were given bronchial asthma model rats by ovalbumin and immune reaction of aluminum hydroxide. After 4 weeks of modeling,isolation of rat serum and lung tissue,the levels of serum IFN-γand IL-4 were tested by Elisa,JAK/STAT signal pathway changed in rat lung tissue by RT-PCR method. Results Compared with Normal Group,Model Group has significantly increased in IL-4 level（ P〈0. 01）,decreased in IFN-γ level（ P〈0. 01）,IL-4/IFN-γ suggests that the immune regulation is significantly unbalanced to the Th2 level,The level of STAT6 and JAK1 mRNA in lung tissue was significantly increased. Compared with Model Group,Medium-dose Chinese herb Group and High-dose Chinese herb Group,have significantly decreased in IL-4 level（ P〈0. 05,P〈0. 01）,increased in IFN-γ level（ P〈0. 05）,The immune balance is obviously regulated in Th1 direction. Lung tissue STAT6 and JAK1 mRNA levels were significantly reduced（ P〈0. 05,P〈0. 01）. Conclusion Bufei Pingchuan decoction can effectively improve the Th1 / Th2 cytokine imbalance in asthmatic rats,reduce STAT6 and JAK1 mRNA expression,regulated the Th1 / Th2 cytokine balance.