焦化废水活性污泥细菌菌群结构分析

Bacterial Community Composition of Activated Sludge from Coking Wastewater

焦化废水是一种高毒难降解的有机废水,以细菌菌群为主的好氧活性污泥决定焦化废水的处理效率,处理焦化废水的活性污泥细菌群落结构鲜见报道.利用454测序技术分析实际焦化废水污泥中的细菌菌群结构和多样性.结果表明,热图聚类分析和主成分分析说明不同焦化废水活性污泥细菌菌群多样性存在差异;焦化废水活性污泥中的细菌门类群主要为Proteobacteria、Planctomycetes、Acidobacteria、Candidatus Saccharibacteria、Bacteroidetes、Cyanobacteria、Actinobacteria、Chloroflexi、Firmicutes、Thaumarchaeota、Ignavibacteriae和Verrucomicrobia,其中Proteobacteria门占主导地位,丰度为36.00%-76.98%;主要属为Thiobacillus、Thauera、Comamonas、Caldimonas、Steroidobacter、Nitrosomonas、Phycisphaera和Gp4,大多数主要属与芳香烃的降解和硝化反硝化过程有关.这些结果为焦化废水污染物的去除机制提供了理论基础.

Coking wastewater is a kind of highly toxic and refractory organic wastewater,and aerobic activated sludge,which is dominated by bacteria,determines the efficiency of coking wastewater treatment. However,the bacterial community structure of activated sludge from coking wastewater has rarely been reported. 454 sequencing technology was applied to investigate the structure and biodiversity of the bacterial community. The results of thermal cluster analysis and principal component analysis demonstrated the differences in biodiversities of different activated sludge bacterial communities. The bacterial communities were mainly composed of Proteobacteria, Planctomycetes, Acidobacteria, Candidatus Saccharibacteria, Bacteroidetes, Cyanobacteria, Actinobacteria,Chloroflexi,Firmicutes, Thaumarchaeota, Ignavibacteriae, Verrucomicrobia and Unclassified bacteria. Proteobacteria was the dominant phylum and its abundance was 36. 00%- 76. 98%. The primary genera were Thiobacillus, Thauera, Comamonas,Caldimonas,Steroidobacter,Nitrosomonas,Phycisphaera and Gp4. Most of these genera were related with aromatic hydrocarbon degradation,nitrification and denitrification processes. These results provide a theoretical basis for removal mechanism of pollutants in coking wastewater.