玻璃化冷冻对卵母细胞IVF受精的影响

Effect of vitrification on IVF of oocytes

目的研究玻璃化冷冻对卵母细胞体外受精能力的影响。方法 IVF周期治疗中受精失败的MII期卵母细胞随机分为玻璃化冷冻组和对照组,对其IVF受精、透明带结合精子、精子透明带穿透以及透明带消化时间和去透明带原核形成等情况进行研究。结果卵母细胞经玻璃化冷冻后正常受精率(24.49%vs.70.00%,P<0.01)和卵裂率(61.22%vs.90.00%,P<0.05)均降低;经0.25%胰酶消化透明带冷冻前后消化时间[(5.95±1.26)vs.(9.51±1.21),P<0.01]和原核形成数目[(5.87±1.23)vs.(4.43±1.21),P<0.05]均存在差异,但透明带结合精子数及去透明带后卵母细胞受精率两组间无统计学差异[分别为(17.32±1.28)vs.(23.14±4.65);87.50%vs.86.36%;P>0.05]。结论卵母细胞玻璃化冷冻不仅影响精子穿透透明带,还影响受精、原核形成及随后胚胎发育的潜力。

Objective To explore the effect of vitrification on in vitro fertilization （IVF） of oocytes. Methods The unfertilized oo- cytes of stage M II in IVF cycles were randomly divided into vitrification group and control group. The time of sperms binding and penetrating zona pellucida, the time of pellucid zone disappearance, and prokaryotic formation without pellucid zone were anlyzed. Results After vitri- fication, the normal fertilization rate and cleavage rate of oocytes decreased significantly （24. 49% vs. 70. 00%, P〈0. 01; 61.22% vs. 90. 00%, P〈0. 05）; after digestion of pellucid zone by 0. 25% pancreatin, there were statistically significant differences in the digestion time and number of prokaryotic formation before and after vitrification [ （5.95±1.26） vs. （9. 51±1.21）, P〈0. 01 ; （5. 87±1.23） vs. （4.43±1.21）, P〈0.05]; there was no statistically significant difference in the number of sperms binding zona pellucida and fertilization rate of oocytes after pellucid zone disappearance [ （17.32±1.28） vs. （23.14±4. 65）, P〉0. 05; 87.50% vs. 86. 36%, P〉0. 05] . Con- clusion Ooeyte vitrification affects ont only sperm penetration through pellucid zone, but also fertilization, prokaryotic formation, and devel- opmental potential of sequent embryos.