RP-HPLC测定油用牡丹籽饼粕和籽壳中单萜苷的含量

Determination of Monoterpene Glycosides in Seed Cake and Seed Shell of Peony for Oil

建立RP-HPIC同时测定油用牡丹籽饼粕和籽壳中6种单萜苷类化合物含量的方法。采用乙腈(A)和磷酸二氢钾缓冲盐(B)(pH=3)为流动相梯度洗脱,梯度为0 min(A,15%)→10 min(A,15%)→22 min(A,25%);检测波长:4"-羟基白芍苷和氧化芍药苷为260 nm,白芍苷、芍药苷、6-O-β-D-吡喃葡萄糖-8-O-苯甲酰基-9α-甲氧基-牡丹酮和白芍苷R1为232 nm,柱温30℃。结果表明,单萜苷类物质在油用牡丹籽饼粕中的含量远远大于在其籽壳中的含量,牡丹籽饼粕可以作为单萜苷类物质的一个重要来源。该方法准确,实用性好,可用于牡丹籽饼粕中单萜苷类成分的定性与定量分析。

To establish an HPLC method for simultaneous determination of 6 monoterpene glycosides in seed cake and seed shell of peony for oil, the separation was performed on an Agilent Zorbax SB C18 column, using acetonitrile （A） and potassium dihydrogen phosphate solution （B） （ pH = 3 ） as the mobile phase by a gradient elution 0 min（A, 15% ）→10 min（A, 15% ）→22 min（A, 25% ）. Detection wavelength was set at 260 nm for the detection of 4＂ - hydroxyl - abiflofin, oxypaeoniflorin, and 232 nm for the detection of abiflofin, paeoniflorin, Paeonidanin, abiflofin R1. Column temperature was set at 30 ℃. The results indicated that the content of monoterpene glycosides in seed cake was higer than that in seed shell, and the seed cake would become the natural resource of monoterpene glycosides. The method was simple, accurate and could be used for quality control of the seed cake of peony for oil.