转Mz_2NHX_1基因拟南芥对盐胁迫的生理响应

Physiological Responses of Mz_2NHX_1 Transgenic Arabidopsis thaliana to Salt Stress

为了探讨珠美海棠Na^+/H^+逆向转运蛋白基因Mz_2NHX_1在植物耐盐中的作用,以野生型拟南芥和转Mz_2NHX_1基因拟南芥为材料,研究不同盐浓度对转基因和野生型拟南芥种子萌发、植株耐盐性相关生理指标的影响。结果表明:在不同盐胁迫下,转基因拟南芥种子发芽率明显高于野生型。随着盐浓度的增加,野生型和转基因植株的电导率呈上升趋势;SOD活性呈先上升后下降趋势;POD活性、可溶性糖含量、脯氨酸含量在野生型植株中呈先上升后下降趋势,在转基因植株中呈不断升高的趋势。盐胁迫下,转基因植株的各项生理指标均优于野生型,表明Mz_2NHX_1基因的过量表达,提高了转基因拟南芥的耐盐性。

To study the role of Na＋/H＋ antiporter gene Mz2NHX1 of Malus zumi in plant salt resistance, wild type Arabidopsis thaliana and transgenic Mz2NHX1 Arabidopsis thaliana were used to study the effects of different salt concentrations on seed germination of transgenic and wild type Arabidopsis thaliana and the physiological characteristics of plant salt resistance. The results showed that the seed germination rate of transgenic A rabidopsis thaliana was significantly higher than that of wild type under different salt stress. With the increase of salt concentration, the conductivity of wild-type and transgenic plants showed an increasing trend; SOD activity in- creased first and then decreased; POD activity, soluble sugar content and proline content increased first and then decreased in wild- type plants, while they were in increasing trend in transgenic plants. The physiological characteristics of transgenic plants were better than that of wild type under salt stress, which indicated that over-expression of Mz2NHX1 gene enhanced the salt tolerance of transgenic A rab idopsis thaliana.