苦马豆根瘤中内生细菌遗传多样性分析

Genetic diversity of endophytic bacteria in nodule of Sphaerophysa salsula

苦马豆(Sphaerophysa salsula)是西北荒漠区重要的豆科植物。本研究采用16SrDNA PCR-RFLP和16SrDNA全序列分析方法,对西北部分地区苦马豆根瘤中内生细菌的遗传多样性及系统发育进行分析。结果显示,115株供试菌株产生了28种遗传图谱类型,对每种图谱类型的代表性菌株进行了16SrDNA全序列分析,其中的根瘤菌分别归属于中华根瘤菌属(Sinorhizobium)、根瘤菌属(Rhizobium)、中慢生根瘤菌属(Mesorhizobium);其余的内生菌分别归属于副球菌属(Paracoccus)、鞘脂单胞菌属(Sphingomonas)、固氮螺菌属(Inquilinus)、假单胞菌属(Pseudomonas)、沙雷氏菌属(Serratia)、分枝杆菌属(Mycobacterium)、诺卡氏菌属(Nocardia)、链霉菌属(Streptomyces)、类芽孢杆菌属(Paenibacillus)、短短芽孢杆菌属(Brevibacillus)、Lysinibacillus、葡萄球菌属(Staphylococcus)和芽孢杆菌属(Bacillus),这表明苦马豆根瘤中内生细菌具有丰富的多样性。

Sphaerophysa salsulais an important legume distributed in northwestern China.In order to better understand the diversity and phylogeny of endophytic bacteria collected from nodules of Swainsonia salsulain different regions of northwestern China,genetic diversity of these isolated endophytic bacteria was estimated using 16 SrDNA PCR-RFLP and 16 SrDNA sequencing.The results showed that these 115 bacteria strains had28 genetic types which were further identified by 16 SrDNA sequencing.These strains belonged to the following genera：Mesorhizobium,Rhizobium,Sinorhizobium,Paracoccus,Sphingomonas,Inquilinus,Pseudomonas,Serratia,Mycobacterium,Nocardia,Streptomyces,Paenibacillus,Brevibacillus,Lysinibacillus,Staphylococcus,Bacillus.These results demonstrated that endophytic bacteria in nodule had rich genetic diversity in S.salsula.