大鼠胃窦黏膜AMP-18mRNA表达减少与胃癌分化程度的关系

Relationship between reduced expressions of AMP-18 mRNA and differentiation degree of gastric cancer

目的：探讨大鼠胃窦黏膜AMP-18的减少或缺失与胃癌分化程度的关系。方法：将SD大鼠分成A、B、C、D 4组（每组5只）,体重160～250 g,用N-甲基-N′-硝基-N-亚硝基胍（MNNG）诱导大鼠胃癌模型。将MNNG用蒸馏水配成100μg/m L的溶液,内含4 mg/L的吐温20及20 mg/L的维生素D3,装入避光饮水瓶中喂饲大鼠8周;第9～16周将MNNG的浓度增至150μg/m L;第17～24周给予180μg/m L的MNNG溶液。14、18、22和24周分别处死4组大鼠进行取材。各组分别进行HE染色切片和Q-PCR法检测大鼠胃窦黏膜AMP-18 m RNA的表达情况。结果：通过参照胃癌分期标准,HE染色切片显示,A、B组胃窦黏膜细胞无明显变化,C组为早期胃癌,D组为进展期胃癌,Q-PCR也显示C组、D组胃黏膜的AMP-18 mRNA表达均明显下降。结论：AMP-18 mRNA在早期和进展期胃癌表达明显下降。

Objective To explore the relationship between the decreased expression of AMP-18 m RNA and different degree of gastric cancer in rats. Methods A total of 20 healthy SD ratswere divided into 4 groups ：groups A, B, C and D. A rat model of stomach cancer was established by feeding water with N-Methyl-N′-NitroNitrosoguanidine（MNNG）, Tween 20 and vitamin D3. The rats in the four groups were killed respectively at weeks 14, 18, 22 and 24. Then gastric antrum mucosa tissues were detected by using HE staining to observe the pathological changes and by real-time quantitative PCR（Q-PCR） technique to detect the expression of AMP-18 m RNA in rats. Results HE staining section showed that group C developed early gastric cancer, and group D developed progressive gastric cancer. The expression of AMP-18 mRNA in groups C and D wassignificantly lower than that in group A by Q-PCR. Conclusion AMP-18 mRNA expression decreased obviously in early orprogressive gastric cancer.