广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响

Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome

目的:研究广藿香油对感染后肠易激综合征(PI-IBS)大鼠肠黏膜机械屏障和免疫屏障的保护作用。方法:将SD大鼠随机分为正常组,PI-IBS模型组、藿香正气液组、广藿香油低、中、高剂量组,每组8只。采用结肠灌注乙酸的方法建立PIIBS大鼠模型。正常组和模型组ig生理盐水,藿香正气液组ig藿香正气液3.3 m L·kg^(-1),广藿香油低、中、高剂量组分别ig广藿香油2,3,4 g·kg^(-1),每日1次,共5 d。采用透射电镜观察结肠黏膜上皮细胞超微结构;采用酶联免疫吸附测定(ELISA)的方法检测各组大鼠血清二胺氧化酶(DAO)和血清免疫球蛋白A(SIg A)含量;采用免疫组化检测结肠肠黏膜细胞间黏附分子-1(ICAM-1)的表达。结果:PI-IBS大鼠结肠上皮细胞微绒毛稀疏,长短不一,有微绒毛断裂现象。广藿香油高剂量组对受损的上皮细胞有改善作用。PI-IBS组血清DAO含量高于正常组(P<0.05)。广藿香油高剂量组血清DAO含量低于PI-IBS组(P<0.05),与正常组比较,无显著性差异。PI-IBS组血清SIg A含量低于正常组(P<0.01)。藿香正气液组、广藿香油高剂量组血清SIg A含量与正常组之间无显著性差异。PI-IBS组ICAM-1蛋白表达IA值明显高于正常组(P<0.01)。广藿香油高剂量组ICAM-1蛋白表达IA值均明显低于模型组(P<0.05),与正常组比较,无显著性差异。结论:广藿香油通过修复受损的肠上皮细胞的超微结构,降低肠道通透性,保护肠黏膜机械屏障。通过促进SIg A分泌,抑制ICAM-1的表达,发挥对PI-IBS免疫屏障的调节作用。

Objective： To protective and regulative effect Patchouli oil on intestinal mucosal mechanical barrier and immune barrier of rats with post-infectious irritable bowel syndrome（ PI-IBS）. Method： SpragueDawley rats were randomly divided into normal control group,PI-IBS model group,Huoxiang Zhengqi liquid group（ HXZQ group）,low-dose of Patchouli oil,middle-dose of Patchouli oil and high-dose of Patchouli oil,with 8 rats in each group. The rat model of intracolonic instillation of acetic acid（ 4%）-induced colitis is set up. Control group and PI-IBS model group were treated with water,HXZQ group was treated with HXZQ（ 3. 3 m L·kg^（-1））.Low-dose of Patchouli oil,middle-dose of Patchouli oil and high-dose of Patchouli oil were treated with Patchouli oil（ 2,3,4 g·kg^（-1））. After different treatment for 5 days,intestinal epithelial ultrastructure was analyzed by transmission electron microscopy（ TEM）,the diamine oxidase（ DAO） activity and SIg A content in serum was measured by ELISA assay. ICAM-1 were detected by immunohistochemistry. Result： Microvilli on the surface of the epithelial cells was sparse,uneven distribution and different length in PI-IBS model group. Recovery effect is obvious in high-dose group of Patchouli oil. Serum DAO content in model group was higher than it of the normal group（ P〈0. 05）. Compared with PI-IBS model group,serum DAO content in high-dose groups were significantly decreased（ P〈0. 05）. The difference between the normal group and high-dose groups was not statistically significant. Serum SIg A content in model group was lower than it of the normal group（ P〈0. 01）. The difference between the normal group and high-dose groups or HXZQ group was not statistically significant. ICAM-1 protein expression IA values in model group was higher than it of the normal group（ P〈0. 01）. Compared with PI-IBS model group,ICAM-1 protein expression IA values in high-dose groups were significantly decreased（ P〈0. 05）.The difference between the normal group and high-dose groups was not statistically significant. Conclusion：Patchouli oil can repair intestinal epithelial ultrastructure in rats with PI-IBS,reduce intestinal permeability,and then protect Intestinal mucosal mechanical barrier. Patchouli oil could promote SIg A,inhibit the expression of ICAM-1,thereby regulate immune barrier of rats with PI-IBS.