氧化去木质素玉米芯同步糖化发酵产2,3-丁二醇

Optimization of 2,3-butanediol production using simultaneous saccharification and fermentation from corncob after delignification by oxidant

2,3-丁二醇是一种重要的平台化合物。选取经过碱性高锰酸钾(APP)预处理后的玉米芯为底物,采用阴沟肠杆菌Enterobacter cloacae CICC10011通过同步糖化发酵工艺(SSF)发酵产2,3-丁二醇。通过对SSF主要工艺参数进行优化,确定最适宜工艺条件为:底物浓度120 g/L,纤维素酶添加量40 FPU/g,木聚糖酶添加量12 000 U/g,发酵温度35℃,初始发酵p H 5.5,转速180 r/min。在最优发酵条件下,以APP预处理后的玉米芯为底物连续发酵36 h,2,3-丁二醇的浓度为21.5 g/L,转化率为0.27 g/g(以纤维素和半纤维素为参照);分别是未处理的玉米芯为底物时的8.41倍和8.71倍。

2,3-butanediol is an important potential platform chemical. In this work,corncob delignified by alkaline potassium permanganate pretreatment（ APP） was used as the substrate to produce 2,3-butanediol by Enterobacter cloacae CICC10011 through simultaneous saccharification and fermentation（ SSF） process. The optimal conditions for SSF were as follows： substrate concentration 120 g / L,cellulase loading 40 FPU / g,xylanase loading 12 000 U / g,initial p H 5. 5,temperature 35 ℃,and the rotate speed 180 r / min. After 36 h fermentation under the optimal SSF conditions,the yield of 2,3-butanediol was 21. 5 g / L and the conversion rate was 0. 27 g / g（ based on cellulose and hemicellulose） with the APP-pretreated corncob as the substrate,which were 8. 41 and 8. 71 times of those with the un-pretreated corncob as the substrate respectively.