辛烯基琥珀酸燕麦β-葡聚糖酯的合成

Synthesis of octenylsuccinate oat β-glucan

近年来,多糖基表面活性剂的合成与功能性质的研究成为食品化学领域比较活跃的研究方向。文中以具有生物活性的燕麦β-葡聚糖为底物,利用辛烯基琥珀酸酐（OSA）为酯化剂,通过优化反应条件,合成辛烯基琥珀酸燕麦β-葡聚糖酯（OSG）。单因素试验结果显示,OSG的取代度随反应时间和OSA添加量的增加而提升,而随反应温度和燕麦β-葡聚糖浓度的增加呈先提升后降低的趋势。在此基础上,响应曲面试验表明,在OSA添加量一定的情况下（0.6 m L）,反应时间（X1）、燕麦β-葡聚糖浓度（X3）以及反应时间（X1^2）、反应温度（X2^2）、燕麦β-葡聚糖浓度（X3^2）的二次项对OSG的取代度有显著影响（P〈0.05）。当反应时间为4.66 h,反应温度为45.6℃,燕麦β-葡聚糖浓度为2.18 mg/m L时,可获得最大取代度（0.038 2）的OSG。^1H-NMR确认了OSG的成功合成。

In recent years,the investigation on synthesis and functional properties of polysaccharide-based macromolecular surfactants became popular in the research field. In this study,octenylsuccinate oat β-glucan（ OSG） was synthesized with bioactive oat β-glucan as substrate and 2-ocentyl succinic anhydride（ OSA） as esterifying agent. The effects of reaction parameters on the degree of substitution（ DS） of oat β-glucan were optimized successively by single-factor experiment and response surface methodology. The results of single-factor experiment revealed that DS of OSG increased with OSA amount and reaction time,and increase and then decrease with reaction temperature and concentration of oat β-glucan. Furthermore,the results of response surface methodology implied that reaction time（ X1）,concentration of oat β-glucan（ X3） and quadratic？ terms of reaction time（ X1^2）,reaction temperature（ X2^2）and concentration of oat β-glucan（ X3^2） showed significant impacts on DS of OSG（ P〈0. 05）. When reaction time,reaction temperature and concentration of oat β-glucan were settled as 4. 66 h,45. 6 ℃ and 2. 18 mg / m L,OSG with the highest DS（ 0. 0382） was obtained. The ^1H-NMR spectra confirmed the successful synthesis of OGS.