超声皂化法提取β-隐黄素

Study on ultrasonic saponification to extract β-cryptoxanthin

以砂糖橘皮粉为原料,采用超声皂化法提取精制β-隐黄素。对比考察了水浴皂化与超声皂化对β-隐黄素得率的影响;考察超声皂化温度、皂化时间、KOH浓度、KOH-乙醇添加量、正己烷添加量等因素对超声皂化反应的影响,对超声皂化反应进行单因素及正交试验,确定超声皂化的最适工艺条件。结果表明:超声皂化与水浴皂化相比能缩短皂化时间,提高β-隐黄素得率;超声皂化最佳工艺条件为,超声时间1 h、超声温度30℃、KOH浓度为1.5 mol/L,正己烷添加量为4 m L,β-隐黄素得率为0.238 4‰。

Ultrasonic saponification was used to extract β-cryptoxanthin from powder of orange peel. Water bath and ultrasonic saponification was compared based on the yield of β-cryptoxanthin. The effects of saponification temperature,saponification time,KOH concentration,KOH-ethanol and n-hexane content on the amount of ultrasound saponification reaction were studied. The optimal conditions for the saponification of β-cryptoxanthin esters were determined by single factor experiment and orthogonal experiment. The results are as follows： compared with water bath saponification,ultrasonic saponification can shorten saponification time,improve β-cryptoxanthin yield. The optimal conditions for ultrasonic saponification are： ultrasonic time1 h,ultrasonic temperature 30 ℃,KOH concentration 1. 5mol / L,n-hexane addition 1∶ 4. Under these optimal conditions,the β-cryptoxanthin yield reached 0. 02384%.