厌食症幼鼠胃窦Cajal间质细胞损伤与健儿散修复作用的实验研究

Effect of Jian'er Powder on ICC of Juvenile Rats with Anorexia

目的:研究厌食症幼鼠胃窦Cajal间质细胞的损伤与健儿散对其的修复作用,从而探讨健儿散干预厌食症的可能机制。方法:40只SPF级SD大鼠幼鼠随机分为模型对照组、正常对照组、健胃消食片对照组和健儿散干预组,正常对照组给予正常饮食,其余3组采用特制饲料喂养法造小儿厌食症动物模型。造模成功后,分别灌胃生理盐水、生理盐水、江中健胃消食片混悬液和健儿散溶液,连续给药4周。观察记录各组大鼠摄食量和体质量的变化,采用透射电子显微镜、免疫组织化学法及RT-PCR法检测各组大鼠胃窦Cajal间质细胞的超微结构、C-kit阳性ICC的数量与分布以及C-kit mRNA的基因表达水平。结果:与模型对照组相比,健儿散干预组的平均摄食量与平均体质量均明显增加(P<0.05),胃窦ICC超微结构的损伤明显修复和改善,Cajal间质细胞c-kit阳性ICC数量显著增加(P<0.05),胃窦C-kit mRNA的基因表达水平显著增加(P<0.05)。结论:健儿散能够增加厌食症幼鼠的摄食量与体质量,修复ICC超微结构的损伤,增加胃窦ICC的表达数量,提高C-kit mRNA基因的表达水平,改善ICC功能并调节其分布,从而达到促进胃肠动力的作用。

Objective：To study the effect of Jian＇er Power on morphological characteristics, quantity and gene expression of 1CC of juvenile rats with Anorexia. And thus to further explore the pathogenesis of infantile anorexia and the effect and mechanism of Jian＇er Power. Methods：A total of 40 SPF grade SD rats ,at the age of 35 -40 days and weighted （65 ±5） g, half male and half female, were randomly divided into 4 groups ： the model group, the normal control group, the Jianwei Xiaoshi Tablet group and the Jian＇er Power group. The normal control group were fed with normal diet and the other 3 groups were given the main causes of anorexia. And then the 3 groups were given the corresponding drugs （Jianwei Xi- aoshi Tablet, Jian＇sr Power, physiological saline）for 4 weeks. Observe and record the rats feeding volume and body weight changes. At the end of the experiment,determine the uhrastructure of ICC via transmission electron microscope and the expression of ICC via Immunohistochemistry and the synthesis of C - kit mRNA via RT - PCR. Results ： The anorectic symptom of the Jianbr Power group SD rats disappeared. The food volume was more than that of the normal control group and Jian＇er Power group（P 〈 0.05）. Compared with the model control group,the uhrastructure of ICC on gastric antrum of the Jian＇er Power group SD rats had significant repaired （P 〈 0.05 ）. Compared with the model control group, the level of C -kit gene expression of the Jian＇er Power group SD rats had obviously increased（P 〈 0.05）. Conclusion：The Jiani＇er Power could obviously increase the food intake and weight and relieve the symptoms of rats with anorexia. The Jian＇er Power could repair and improve the damage of ultrastructure of ICC of rats with anorexia. Jiane＇r Power could improve the expression level of C - kit positive ICC of rats with anorexia and increase the number of ICC. Jiane＇r Power could increase the synthesis of C - kit mRNA in gastric antrum of rats with anorexia.