喀西茄WRKY基因的分离及其受黄萎病诱导的表达分析

Expression Analysis of WRKY Transcription Factor Isolated from Solanum aculeatissimum Induced by Verticillium dahliae

利用已获得的喀西茄(Solanum aculeatissimum)接种黄萎病前后的根部转录组数据库,前期筛选得到了4个与植物WRKY转录因子蛋白一致性较高的Unigene序列。系统进化分析表明,这4个转录因子均含有典型的WRKY结构域,分别属于WRKY蛋白家族的第IC类、IIa类和III类。利用实时荧光定量PCR技术分析了这4个WRKY基因在喀西茄不同组织(根、茎和叶)以及接种黄萎病后根部不同时期的表达情况,结果表明,Unigene6502和Unigene20920在根部的表达量显著高于茎部和叶片,而CL1273.Contig2和CL3641.Contig1在叶片的表达量显著高于根部和茎部。这4个WRKY基因在喀西茄根部接种黄萎病菌后具有不同的表达模式,Unigene6502和Unigene20920接种后呈先上调后下调的表达模式,CL1273.Contig2和CL3641.Contig1分别呈上调和下调的表达模式。

A total of four unigene sequences that share high homology with WRKY protein were previously obtained from the root transcriptome database of Solanum aculeatissimum.Phylogenetic analysis showed that the four WRKY transcription factors belong to IC,IIa,and III group of WRKY family,respectively.The expression profiles of these four WRKY genes in different organs（root,stem and leaf）,as well as in the root of Solanum aculeatissimum of different time after Verticillium dahliae inoculation were analyzed by quantitative real-time PCR.The results showed that the expression levels in root of Unigene6502 and Unigene20920 were significant higher than those in stem and leaf,but the expression levels in leaf of CL1273.Contig2 and CL3641.Contig1 were significantly higher than those in root and stem.The four WRKY genes have different expression patterns upon Verticillium dahliae inoculation.The expression of Unigene6502 and Unigene20920 were up-regulated and then was significantly decreased,while the expression of CL1273.Contig2 and CL3641.Contig1 were up-regulated and down-regulated,respectively.