扩增子测序技术分析红茶菌中优势微生物的研究

Study on Analysis of the Dominant Microorganisms in Kombucha by the Technology of Amplicon Sequencing

红茶菌作为一种传统健康保健饮品,由于发酵过程中需要多种微生物的参与,因此需要对混合菌种进行高效的分离鉴定。本文通过提取红茶菌中的基因组DNA,采用高通量测序的方法对16S rDNA和ITS的扩增子进行测序,实现对红茶菌中优势微生物的分析鉴定。通过对可操作分类单元进行丰度分析和物种分类树统计,最终得到主要的细菌组成为:醋酸菌89.11%,拟杆菌5.56%,颤螺旋杆菌1.77%;优势真菌组成为:酵母菌为93.48%,其中嗜酒假丝酵母61.76%,酵母菌Blastobotrys adeninivorans 30.97%;α多样性分析结果显示:测序数据较为合理,测序数据量大,可以反映样品中绝大多数的微生物物种信息。

Study on the application of the amplification sequencing technology in the research of compound bac-teria fermented beverage. Taking the Kombucha beverage as an example,throught the genomic DNA extraction in oral liquid of Kombucha,amplicon sequencing of 16S rDNA and ITS using the method of high-throughput se-quencing of amplicons of sequencing to analysis and identification the dominant microbial in oral liquid of Kom-bucha. The classification and statistics of the species by the special species classification tree,finally got the main bacteria composition：Acetobacter was 89.11%, Bacteroides was 5.56%,Oscillospira was 1.77%. The main fungus composition：Yeast was 93.48 %,of which Candida ethanolica was 61.76 %, Blastobotrys adeninivorans was 30.97%. The result of Alpha Diversity analysis showed that：the sequencing data was more reasonable,the sequencing data amount was large enough,can reflect most of the microbial species information in the sample. The the application of the amplification sequencing technology has advantages in the analysis of the advantages of the compound bacteria fermented beverage and the detection of the content of probiotics.