芝麻花粉管介导胰岛素基因的遗传转化

Insulin gene transformation into sesame via pollen-tube pathway

为建立芝麻油体表达体系这一新型植物生物反应器,采用花粉管通道法,将含有胰岛素基因的双元表达载体PBI121质粒转入冀黑芝1号芝麻。对转化株后代进行卡那霉素筛选、PCR扩增及PCR-Southern杂交检测。结果显示:胰岛素基因已整合到受体芝麻的基因组DNA中,并获得了T_5转化株,胰岛素基因可以遗传到第5代转化株。说明芝麻花粉管介导胰岛素基因的遗传转化油体表达体系可行。

To establish a novel plant bioreactor based on sesame oil body expression system, the binary vector PBI121 containing the insulin gene was transformed into sesame Jiheizhi No. 1 via pollen-tube pathway. The transformed plant with Kanamycin resistance was confirmed by PCR amplification and PCR-Southern hybridization. The results showed the insulin gene was integrated into the genome of sesame seeds, and could be passed onto generation T5 . In conclusion, it is feasible to genetically transform insulin gene into sesame oil body via a pollen-tube mediated pathway.