茉莉酸合成关键酶基因FaOPR3调控草莓果实成熟

Regulation of strawberry fruit ripening by jasmonic acid synthesis gene FaOPR3

为了研究茉莉酸(Jasmonic acid,JA)合成路径关键酶基因FaOPR3在草莓果实成熟中的作用,测定了八倍体红颜草莓果实成熟过程中内源JA含量,并用外源茉莉酸甲酯(Me JA)涂抹草莓果实观察其果实着色情况及测定相关酶基因的表达,同时使用瞬时基因表达对草莓果实进行FaOPR3基因的超表达和干扰。结果显示草莓果实中内源JA含量从小绿果期到白果期急剧上升,到果实成熟后开始下降。草莓果实涂抹外源JA,能够促进果实发育和成熟。FaOPR3基因的过量表达可诱导果实内源JA含量的增加,并且可诱导色素合成基因FaCHS、FaCHI、FaF3H、FaUFGT、FaDFR的表达量上升。而FaOPR3基因干扰后果实内源JA含量降低,并且抑制相关色素代谢基因表达。说明FaOPR3基因能够促进草莓果实着色和成熟。

To study the role of jasmonic acid （ JA） synthesis key enzyme gene FaOPR3 in strawberry fruit develop-ment, the endogenous JA content in the octoploid strawberry Benihoppe （Fragaria＆#215;ananassa Duch.） during fruit ripening were measured ,the fruit coloring was observed and related genes expression levels were detected. The endogenous JA con-tent increased dramatically from small green fruit period to white fruit period, peaked at white fruit period, and dropped quickly afterwards. Exogenous MeJA treatment promoted strawberry fruit development and ripening. Transient gene expres-sion revealed that FaOPR3 over-expression induced the increase of endogenous JA content in fruit and pigment metabolism-associated genes expression levels, such as FaCHS,FaCHI,FaF3H,FaUFGT and FaDFR genes. On the contrary, FaOPR3 gene interference reduced JA content and inhibited pigment metabolism-related genes expressions. It is indicated that FaO-PR3 gene plays an important role in strawberry fruit ripening, promoting strawberry fruit coloring and ripening.