芒果三芪肺纤方对博莱霉素致小鼠肺纤维化作用研究

Effect of Mangguosanqi Feixian Prescription on bleomycin-induced pulmonary fibrosis in mice

目的观察芒果三芪肺纤方对博莱霉素诱导小鼠肺纤维化的改善作用。方法昆明小鼠随机分为对照组,模型组,地塞米松(阳性药,1 mg/kg)组,芒果三芪肺纤方高、中、低剂量(以生药计5.00、3.30、1.65 g/kg)组,通过鼻腔1次性滴入6 mg/kg盐酸博莱霉素制备小鼠肺纤维化模型,对照组滴入等体积的生理盐水。于造模后第14天开始ig给药,每天给药1次,连续给药14 d后处死小鼠,试剂盒法测定肺组织匀浆中的羟脯氨酸(HYP)、丙二醛(MDA)、白介素-1β(IL-1β)水平和超氧化物岐化酶(SOD)活力;HE染色观察肺组织病理切片,Masson染色观察胶原纤维的分布情况。结果与模型组比较,芒果三芪肺纤方高、中、低剂量组小鼠HYP和IL-1β水平均显著降低(P<0.01),SOD活力显著提高(P<0.05、0.01),高和中剂量组小鼠MDA水平显著降低(P<0.05、0.01);HE和Masson染色显示,芒果三芪肺纤方组肺泡炎损伤和肺纤维化病变程度明显减轻(P<0.01),高剂量组效果最好。结论芒果三芪肺纤方通过提高SOD活力、降低MDA水平,发挥抗自由基损伤作用;降低HYP的水平,减少纤维蛋白的形成;降低IL-1β水平,发挥抗炎作用;显著减轻肺泡炎症损伤、抑制肺纤维化病变程度,对博莱霉素诱导的小鼠肺纤维化具有很好的改善作用。

Objective To investigate the effect of Mangguosanqi Feixian Prescription on bleomycin-induced pulmonary fibrosis in mice. Methods Kunming mice were randomly divided into six groups： control group, model group, dexamethasone（1 mg/kg, positive drug） group, Mangguosanqi Feixian Prescription low, medium and high dose（5.00, 3.30, and 1.65 mg/kg） groups. Mice were prepared with intranasal instillation infusion with 6 mg/kg bleomycin, control group treated with the same volume of physiological saline. Mice in each treatment group were given appropriate intervention 14 d after modeling, and were sacrificed after 14 d. Reagent kit method was used to determine the levels of homogenate（HYP）, malondialdehyde（MDA）, and interleukin-1β（IL-1β）, and the activity of superoxide enzyme（SOD） in lung tissue homogenate. Pathological sections were stained with haematoxylin-eosin and Masson dyeing for pathological diagnosis. Results Compared with model group, the level of HYP, IL-1β in Mangguosanqi Feixian Prescription low, medium, and high dose groups was decreased significantly, the activity of SOD was increased significantly, and the levels of MDA in medium and high dose groups were decreased significantly（P 0.05, 0.01）. Mangguosanqi Feixian Prescription group could significantly relieve inflammation and restrain the degree of pulmonary fibrosis（P 0.01）. High-dose group is the best. Conclusion Mangguosanqi Feixian Prescription can increase SOD activity, decrease MDA levels play against free radical injury, reduce the content of HYP, reduce the formation of fibrin, and regulate the expression of IL-1β to exert anti-inflammatory effects. It can also significantly reduce damage alveolitis and inhibition of pulmonary fibrosis disease. It has a very good therapeutic effect on bleomycin-induced pulmonary fibrosis in mice.