两种甲型H1N1流感病毒核酸检测方法比较研究

Comparison to application of conventional PT-PCR and fluorescence quantitative RT-PCR in detecting influenza A virus(H1N1)

目的比较荧光定量RT-PCR和常规的RT-PCR方法检测甲型H1N1流感病毒。方法同时采用荧光定量RTPCR和常规的RT-PCR方法针对天津市东丽区所属医院2014-2015年收集的流感样症状患者的鼻咽拭子样本进行检测。结果研究期间该区所属医院共收集263例收集的流感样症状患者的鼻咽拭子样本。采用荧光定量RT-PCR方法在其中26例标本中发现H1N1流感病毒RNA;采用常规的RT-PCR方法在其中25例标本中发现H1N1流感病毒RNA。两种检测结果的一致率为96.15%。结论常规RT-PCR和荧光定量RT-PCR在检测甲型H1N1流感病毒中一致率很高。

Objective To compare application of conventional PT-PCH and iluorescenc6 quantitative Ill - PCR in detecting new type H1N1 influenza virus. Methods Specimens from the upper respiratory pathways of patients presenting with influenza-like-illness were collected at hospitals in Dongli district, Tianjin between 2014-2015. All collected specimens were tested by conventional PT-PCR fluorescence quantitative RT-PCR. Results 263 pieces of sample collected in the district were tested by the two methods. 26 were positive for H1N1 by fluorescence quantitative RT-PCR, and 25 by conventional RT-PCR. The concordance rate of the two methods was as high as 96.15%, Conclusion High concordance rate was found in the rapid detection of influenza A virus （H 1N 1 ） by conventional RT-PCR and fluorescence quantitative RT-PCR.