UPLC-Q-TOF/MS技术结合诊断离子方法快速分析连钱草中黄酮类化合物

Rapid Analysis on Flavonoids in Glechoma longituba(Nakai)Kupr by UPLC-Q-TOF/MS Couple with Diagnostic Ions

建立了超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术结合诊断离子法快速分析连钱草中黄酮类化合物。采用信息依赖型的扫描(IDA)模式,在电喷雾负离子模式下分析黄酮标准化合物的裂解规律及特征碎片离子信息,并以标准物质的特征碎片离子作为诊断离子,全面鉴别连钱草中的黄酮类成分。在连钱草中共鉴别出35个黄酮类化合物,其中有25个化合物为在该植物中首次发现;除白杨素、芹菜素、毛蕊异黄酮、木犀草素、山奈酚、槲皮素外,其他化合物均以黄酮苷的形式存在。该方法可为连钱草的质量控制及药效学研究提供技术支持。

With the modernization of Chinese medicine, the development of effective quality control method for Chinese herbs is one of the key scientific problems in the future. A method of high performance liquid chromatography-time-of-fight mass spectrometry （UPLC-Q-TOF/MS） couple with diagnostic ions under negative mode of ESI was developed for analyzing flavonoids in Glechoma longituba （Nakai） Kupr. An information dependent acquisition （IDA） mode was used to acquire high-precision mass spectrometric data for comprehensive profiling of constituents from Glechoma longituba （Nakai） Kupr. Both precursor ions and corresponding fragment ions （mass deviation are less than 5 × 10^-6） were simultaneously generated in negative mode. The precursor ions showing chemical formula could provide candidate compounds by querying the relevant database. Some database, such as Massbank, SciFinder, Chemspider, were used to quickly screen the possible compounds in Glechoma longituba （Nakai） Kupr. The characteristic groups and structure information were obtained by analyzing fragment ions, and that was very important to select out target ingredients form candidate compounds. The results show that a total of 35 flavonoids are identified, and 25 of which flavonoids are identified for the first time from Glechoma longituba （Nakai） Kupr. In addition to chrysin, apigenin, isoflavone, luteolin, kaempferol and quercetin, all the other compounds are flavonoid glycosides. The method can provide the technical support and material basis for pharmacodynamics research and quality control of Glechoma longituba （Nakai） Kupr.