miRNA-296对人类风湿关节炎滑膜成纤维细胞侵袭能力影响的体外实验研究被引量：1

The effect of miRNA-296 on the invasive ability of human rheumatoid synoviocyte in vitro

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摘要目的探讨微小RNA（miRNA-296）对人类风湿关节炎（RA）滑膜成纤维细胞MH7A的影响及机制。方法将miRNA-296抑制物转染人人类风湿关节炎滑膜成纤维细胞MH7A细胞株后，采用Transwell小室法检测细胞侵袭能力变化；实时定量荧光聚合酶链反应（Real-timePCR）检测转染miRNA-296抑制物前后Janus激酶2（JAK2）、信号转导子和转录激活子3（STAT3）、瘦素的mRNA表达变化；Westernblot技术分析miRNA-296抑制物对MH7A细胞中JAK2、STA33、瘦素蛋白的表达影响。结果miRNA-296抑制物对MH7A细胞侵袭有明显的抑制作用。下调miRNA-296能够抑制MH7A细胞中JAK2、STAT3mRNA和蛋白的表达、提高瘦素mRNA和蛋白的表达。结论低表达miRNA-296可以显著抑制人类风湿关节炎滑膜成纤维细胞MH7A的侵袭能力，这可能与JAK2／STAT3／瘦素信号通路有关。 Objective To explore the effect of microRNA-296 （miRNA-296） on human rheumatoid synoviocyte MH7A and its mechanism. Methods MiRNA-296 inhibitors were transfected into MH7A ceils. The cell invasion capability was determined by transwell chamber assay. The expression of miRNA-296 was detected by real-time PCR. The expression of JAK2/STAT3/leptin mRNA were examined by real-time PCR and their protein levels were examined by Western blotting. Results MiRNA-296 inhibitors significantly inhibited the invasion of MH7A cells. MiRNA-296 inhibitors could inhibit the mRNA and protein expression of JAK2 and STAT3 and enhanced the mRNA and protein expression of leptin. Conclusion Low expression of miRNA-296 significantly inhibits the invasion ability of MH7A cells by JAK2/STAT3/leptin pathway.

作者王萱怡张育魏华李国青柳桂萍王雯雯刘延庆

机构地区扬州大学临床医学院(苏北人民医院)风湿免疫科扬州大学医学院中西医结合学系

出处《临床内科杂志》 CAS 2016年第10期702-705,共4页 Journal of Clinical Internal Medicine

基金国家自然科学基金资助项目（81274141、81450051、81573656、81403232）江苏省自然科学基金面上项目（BK20141280）江苏省中医药科技项目（LZ11210）江苏省苏北人民医院院级项目（yzue-ms201409）江苏省研究生培养创新工程项目（KYZZ15_0368）

关键词 miRNA-296 JANUS激酶2 信号转导子和转录激活子3 瘦素类风湿关节炎 miRNA-296 JAK2 STAT3 Leptin Rheumatoid arthritis

分类号 R593.22 [医药卫生—内科学]

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