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农杆菌转化体系在药用微藻中的研究进展 被引量:1

Research progress on the Agrobacterium-mediated medicinal microalgae genetic transformation system
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摘要 农杆菌介导的遗传转化具有操作简便,转化率高,可低拷贝和大片段整合外源基因在宿主染色体上等优点,已经在植物中广泛应用。微藻作为一种重要的种质资源,是多种功能活性产物的初级生产者,在药物开发方面极具发展潜能。农杆菌介导的转化体系在部分微藻中获得成功,有效提高了药用活性产物的生产效率,改良了微藻的生理性能,为微藻药用资源的大规模工业化生产奠定了基础。本文综述了微藻来源的药用功能产物及农杆菌转化体系在微藻中的研究现状和影响转化效率的因素,并展望了该技术在药用微藻中的应用前景,为药用微藻资源的基因工程改造提供一定的参考和借鉴意义。 Agrobacterium-mediated genetic transformation has been widely used for plants, owing to its merits such as operation simplicity, high efficiency of transformation, and transferring a large segment of foreign DNA with low copy of the transgene(s) into host chromosomes. Microalgae are known as germplasm resources and primary producers of a variety of functionally activated products. It also possesses great potential in the development of pharmaceutical exploitation. Agrobacterium-mediated genetic transformation system has been established successfully in some microalgae. This technology has efficiently increased the productivity of medicinal activated products and improved the physiological performance in microalgae, thus laying foundation for the mass production of medicinally valuable microalgae at large-scale. This paper summarizes the medicinally activated products from microalgae, the research progress on the Agrobacterium-mediated genetic transformation in microalgae as well as the factors affecting transformation efficiency, the application prospect of this technique in medicinal microalgae, and ultimately providing certain reference to the genetic engineering of medicinal microalgae.
作者 王柳英 林祥志 娄素琳 王昭凯 WANG Liu-ying LIN Xiang-zhi LOU Su-lin WANG Zhao-kai(Engineering Research Center for Comprehensive Utilization of Marine Biological Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen Fujian 361005 School of Life Sciences, Xiamen University, Xiamen Fujian 361005)
出处 《中南药学》 CAS 2016年第10期1084-1089,共6页 Central South Pharmacy
基金 海洋公益性行业科研专项经费项目(No.201505032) 厦门南方海洋研究中心项目(No.14GZP021NF21)
关键词 微藻 药用产物 农杆菌介导 遗传转化 转化效率 microalgae medicinal product Agrobacterium-mediated genetic transformation transformation efficiency
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