髓核细胞凋亡调控中的MicroRNA-182

Regulatory function of microRNA-182 in apoptosis of nucleus pulposus cells

背景:前期从中发现hsa-mi R-182可能与髓核细胞中凋亡相关基因细胞色素C(cytochrome C,Cycs)基因及钙调磷酸酶(calcineurin)的C亚基Cn B(PPP3R1)基因有密切联系。目的:以质粒为载体,将mi R-182及细胞色素C和PPP3R1基因转染进髓核细胞系,检测细胞系中凋亡相关基因细胞色素,明确C及PPP3R1的表达量miR-182是否参与髓核细胞凋亡的调控。方法:miR-182进行生物信息学预测,将其与预测得到的目标基因进行质粒合成并转染髓核细胞,并建立空白对照,最后进行细胞裂解检测目标基因表达情况。结果与结论:(1)经检测,miR-182较空白对照组髓核细胞对细胞色素C基因的表达有明显的抑制作用(P<0.05);(2)与空白对照组比较,miR-182对于PPP3R1基因的表达无抑制作用。(3)结果表明,miR-182对于髓核细胞凋亡相关基因细胞色素C有明显抑制作用,可能参与髓核细胞凋亡的调控。

BACKGROUND：Previous study has found that hsa-miR-182 is probably related to the apoptosis-related genes such as cytochrome C （Cycs C） and calcineurin subunit CnB （PPP3R1） in nucleus pulposus cells. OBJECTIVE：To determine whether miR-182 plays a regulatory role in nucleus pulposus cel apoptosis by detecting the relative gene expression levels after transfecting miR-182 with Cycs C and PPP3R1 into nucleus pulposus cel s via plasmid delivery. METHODS：After a bioinformatics prediction about miR-182, miR-182 and target genes were transfected into the nucleus pulposus cel s, and at the same time, blank control group was established. Then the expression levels of the target genes were detected through cel lysis. RESULTS AND CONCLUSION：miR-182 significantly inhibited the expression of Cycs C in nucleus pulposus cel s compared with the blank control group （P〈0.05）. Compared with the blank control group, miR-182 made no inhibitory effect on the expression of PPP3R1. These findings suggest that miR-182 may play a regulatory part in nucleus pulposus cel apoptosis by inhibiting the expression of Cycs C.