慢性膀胱炎相关膀胱过度活动症大鼠模型的建立

Establishment of Over Active Bladder Model Associated with Chronic Cystitis in Rat

目的 建立一种发病机制、尿流动力学表现与临床相近的慢性膀胱炎相关膀胱过度活动症（OAB）大鼠模型.方法 将40只雌性SD大鼠随机分为模型组和生理盐水对照组;模型组以大肠埃希氏菌膀胱灌注,3d后搜集尿液常规显微镜检查,证实膀胱炎并以庆大霉素灌胃每日4次,连续7d,第10日复查尿常规,每隔10d为1个周期,经4个周期后行尿流动力学检查及膀胱HE染色病理学检查.生理盐水对照组用等量生理盐水代替大肠埃希氏菌溶液,其余处理同模型组.2组同条件饲养.结果 模型组膀胱标本可见少量上皮组织退变、凋亡、脱落,黏膜固有层见散在的淋巴细胞,行尿流动力学检查确认13只建模成功.结论 通过大肠埃希氏菌ATCC35218反复膀胱灌注诱导SD大鼠慢性膀胱炎,最终可建立慢性膀胱炎相关OAB大鼠模型,为临床上常见的膀胱炎后OAB的发病机制及治疗、研究提供思路.

Objective To build the over active bladder （OAB） related to chronic cystitis model which is similar to clinical process including pathogenesis and urodynamic performance. Methods Forty female SD rats were randomly divided into model group and saline control group. Model group： the bladders were instilled with Escherichia coli ATCC35218 （1 ×10^8-1.2 × 10^8CFU/mL） 100 μL, and a urine test were done after 3 days to confirm cystitis. The rats were given by gavage with Gentamicin （0. 5 mL, 2 million units） 4 times a day × 7 days and urine were retested 10 days a cycle. The rats were done urodynamics and HE staining for bladder tissue after 4 cycles. Saline control group： were given by gavage with the same amount of saline solution instead of Escherichia coli, the remaining process was the same as the model group. The animals were raised under the same conditions. Results A small amount of epithelial was found degeneration, apoptosis, loss, and scattered lymphocytes in the lamina propria in model group. Thirteen rats were successfully modeled. Conclusion The OAB animal model can be established after cystitis by repeated E.coli infusion into bladder of female SD rats＇. It offers ideas for pathogenesis, treatment and research about OAB associated with chronic cystitis.