芪蓟肾康颗粒总黄酮对大鼠肾小球系膜细胞外基质动态平衡的干预实验

Mechanism Analysis of Qiji Shenkang Total Granule Flavonoids on Regulating Glomerular Extracellular Matrix Dynamic Equilibrium through Inhibition of Inflammatory Response

目的 通过体外肾小球系膜细胞培养实验,明确芪蓟肾康颗粒总黄酮是否为组方的有效组分,探讨其作用机制.方法 运用血管紧张素Ⅱ诱导体外培养的肾小球系膜细胞,使细胞外基质增加,经芪蓟肾康颗粒总黄酮干预后,观察白细胞介素-6（IL-6）、单核细胞趋化蛋白-1（MCP-1）、纤维粘连蛋白（FN）和Ⅳ型胶原（Col Ⅳ）的蛋白表达.结果 与Ang Ⅱ组比较芪蓟肾康颗粒总黄酮可降低IL-6、MCP-1的蛋白表达,FN和Ⅳ型胶原的含量也都分别降低（P＜0.01）.结论 芪蓟肾康颗粒总黄酮可能是通过调节促炎症因子的表达,从而抑制细胞外基质过度积聚.

Objective Through in vitro mesangial cell culture experiments, to explore if the total flavonoids of Qiji Shenkang Granule is the key effective component in the Fufang, and to further explore its mechanism. Method Used the macroporous resin adsorption method to extract total flavonoids in Fufang, and then use vascular tension angiotensin II to induce apoptosis in cultured mesangial cell membrane, which increased the extracellular matrix, after the intervention of total flavonoids of Qiji Shenkang Granule, analyzed the effect of intefleukin 6 （IL-6） and monocyte chemotaxis protein- 1 （MCP- 1）, fibronectin （FN） and type IV collagen （CollV） protein expression. Results the Qiji Shenkang Granule flavonoids in high, dose group significantly reduced IL-6 and MCP-1 protein expression, FN and collagen content were also reduced, compared with Ang II group, P〈0.01. Conclusion By regulating the expression of pro-inflammatory factors, the total flavonoids in the Qi Ji Shen Kang Granule can inhibit the excessive accumulation of extracellular matrix.