宫颈病变患者高危型人乳头瘤状病毒感染与血清白细胞介素1β、白细胞介素2、白细胞介素10的关系

The relationship between cervical lesions in patients with high -risk human papilloma virus infection and serum interleukin-1 beta,interleukin-2,interleukin-10

目的：探讨宫颈病变患者高危型人乳头瘤状毒（hr-HPV）感染与患者血清白细胞介素1β（IL-1β）、白细胞介素2（IL-2）、白细胞介素10（IL-10）的关系。方法以180例宫颈病变患者为观察对象，其中感染 HPV 组105例，未感染 HPV 组75例，观察两组血清 IL-1β、IL-2、IL-10水平，比较不同 hr-HPV DNA 负荷量患者的血清 IL-1β、IL-2、IL-10水平，分析 hr-HPV DNA 负荷量与 IL-1β、IL-2、IL-10水平的相关性。结果感染 HPV 组 IL-1β、IL-10、IL-2分别为（0．85±0．23）ng／L、（182．35±10．02）ng／L、（38．97±5．23）ng／L，未感染 HPV 组分别为（0．62±0．18）ng／L、（305．42±11．13）ng／L、（25．18±3．16）ng／L，感染 HPV 组 IL-1β和 IL-10水平明显高于未感染 HPV 组，IL-2水平明显低于未感染 HPV 组，差异均有统计学意义（t ＝7．222、20．328、－7．558，均 P ＜0．01）；不同病变程度宫颈病变者 hr-HPV DNA 负荷量差异有统计学意义，且随着病变程度的增加 hr-HPV DNA 负荷量明显增加（t ＝6．214、19．097、33．906、6．952、6．274，均 P ＜0．05）；IL-1β和 IL-10水平由高到低分别为 hr-HPV DNA 负荷量＞1000者、100～1000者和＜100者，IL-2水平由高到低分别为 hr-HPV DNA 负荷量＜100者、100～1000者和＞1000者；宫颈病变合并 HPV 感染患者的 hr-HPV DNA 负荷量与患者血清 IL-1β和 IL-10水平正相关（r ＝0．452、0．422，P ＝0．035、0．019），与 IL-2水平负相关（r ＝－0．398，P ＝0．027）。结论宫颈病变患者 hr-HPV 感染与患者血清 IL-1β、IL-2、IL-10水平密切相关，可将患者的 IL-1β、IL-2、IL-10水平作为临床监测的重要指标。

Objective To explore the relationship between cervical lesions in patients with high -risk human papilloma virus（HPV）infection and serum interleukin -1 beta（IL -1 ）,interleukin 2（IL -2）,interleukin 10（IL -10）.Methods 180 cases of cervical lesions were treated in our hospital from August 2013 to August 2015. Among them,105 cases were infected with HPV,and 75 cases were not infected by HPV.The serum levels of IL -2, IL -10 and IL -1βwere observed in two groups,and compared the levels of serum IL -2,IL -10 and IL -1βin patients with different DNA hr -HPV load,the correlation between DNA hr -HPV load and IL -1β,IL -2 and IL -10 levels was analyzed.Results The levels of IL -1β,IL -10,IL -2 were （0.85 ±0.23）ng/L,（182.35 ± 10.02）ng/L,（38.97 ±5.23）ng/L in patients with HPV infection,the levels of IL -1β,IL -10,IL -2 in patients without HPV infection were （0.62 ±0.18）ng/L,（305.42 ±11.13）ng/L,（25.18 ±3.16）ng/L.The levels of IL -1β,IL -10 were significantly higher in HPV group than in without HPV infection group,the level of IL -2 in HPV group was significantly lower than that of uninfected HPV group,and the differences were statistically significant （t =7.222,20.328,-7.558,all P 〈0.01）.There were significant differences in the load of DNA hr -HPV between different pathological changes of cervical lesions,and the DNA hr -HPV load increased significantly with the increase of the degree of disease（t =6.214,19.097,33.906,6.952,6.274,all P 〈0.05）.IL -1βand IL -10 levels from high to low were DNA hr -HPV load volume 〉1 000,100 -1 000 and 〈100,IL -2 levels from high to low were DNA hr -HPV load 〈100,100 -1 000 and 〉1 000.DNA hr -HPV load was positively correlated with serum IL -1βand IL -10 levels in patients with cervical lesions and HPV infection（r =0.452,0.422,P =0.035,0.019）,and nega-tively correlated with IL -2 level（r =-0.398,P =0.027）.Conclusion hr -HPV infection is closely correlated with serum IL -1β,IL -2,IL -10 levels in cervical lesions patients,IL -1,IL -2 and IL -10 can be used as impor-tant index for clinical monitoring.