胃癌患者血清miRNA-223的表达水平及其诊断价值

Diagnostic value and expression of serum miRNA-223 in patients with gastric cancer

目的：探讨胃癌患者血清中miRNA‐223的表达水平及其诊断价值。方法：采用实时定量PCR法检测50例胃癌患者和50名健康对照者血清miRNA‐223的表达量，分析miRNA‐223与胃癌临床病理参数的关系。构建受试者工作特征（ROC）曲线，比较miRNA‐223与癌胚抗原（CEA）的诊断效能。结果：胃癌患者血清miRNA‐223水平高于健康对照组（P＜0．05）。ROC曲线显示，miRNA‐223诊断胃癌的曲线下面积（AUC）达0．636（95％ CI 0．526～0．746），灵敏度、特异度分别为48％、82％；miRNA‐223联合CEA诊断胃癌的 AUC达0．769（95％ CI 0．675～0．862），灵敏度、特异度分别为70％、78％。结论：miRNA‐223联合CEA有助于胃癌的诊断。

Objective：To discuss the expression and diagnostic value of miRNA‐223 in patients with gastric cancer . Methods：The expression of serum miRNA‐223 was detected in 50 patients with gastric cancer and 50 healthy controls by using real‐time quantitative reverse transcription polymerase chain reaction （qRT‐PCR ） . The relationship of miRNA‐223 and clinicpathological parameter of gastric cancer was analyzed . The receiver operating curve （ROC ） was constructed and the diagnostic value of miRNA‐223 and carcinoembryonic antigen （CEA） was compared .Results： The serum miRNA‐223 in patients with gastric cancer was significantly higher than control group .The ROC indicated that area under the ROC curve （AUC） of miRNA‐223 was 0 .636 （95% CI 0 .526‐0 .746） with sensitivity of 48% and specificity of 82% while AUC of combination of miRNA‐223 and CEA was 0 .769 （95% CI 0 .675‐0 .862） with sensitivity of 70% and specificity of 78% . Conclusions：Combining miRNA‐223 and CEA can be used to diagnosis gastric cancer .